caa a22 4500 606207546 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2296-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2296-2 OsKASI, a β-ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice ( Oryza sativa L.) [Elektronische Daten] [Wona Ding, Li Lin, Botao Zhang, Xianbo Xiang, Jing Wu, Zhichong Pan, Shihua Zhu] Main conclusion : The involvement of OsKASI in FA synthesis is found to play a critical role in root development of rice. The root system plays important roles in plant nutrient and water acquisition. However, mechanisms of root development and molecular regulation in rice are still poorly understood. Here, we characterized a rice (Oryza sativa L.) mutant with shortened roots due to a defect in cell elongation. Map-based cloning revealed that the mutation occurred in a putative 3-oxoacyl-synthase, an ortholog of β-ketoacyl-[acyl carrier protein] synthase I (KASI) in Arabidopsis, thus designated as OsKASI. OsKASI was found to be ubiquitously expressed in various tissues throughout the plant and OsKASI protein was localized in the plastid. In addition, OsKASI deficiency resulted in reduced fertility and a remarkable change in fatty acid (FA) composition and contents in roots and seeds. Our results demonstrate that involvement of OsKASI in FA synthesis is required for root development in rice. Springer-Verlag Berlin Heidelberg, 2015 Fatty acid synthesis nationallicence Map-based cloning nationallicence Oryza nationallicence OsKASI nationallicence Root elongation nationallicence Short root nationallicence ACCase : Acetyl-CoA carboxylase nationallicence ACP : Malonyl-acyl carrier protein nationallicence CoA : Malonyl-coenzyme A nationallicence ENR : Enoyl-ACP reductase nationallicence FA : Fatty acid nationallicence FAS : Fatty acid synthase nationallicence GFP : Green-fluorescent protein nationallicence GUS : β-Glucuronidase nationallicence KAS : β-Ketoacyl-[acyl carrier protein] synthase nationallicence STS : Sequence-tagged site nationallicence VLCFAs : Very-long-chain fatty acids nationallicence WT : Wild type nationallicence Ding Wona College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut Lin Li School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut Zhang Botao Department of Botany, La Trobe University, Melbourne, VIC, Australia aut Xiang Xianbo School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut Wu Jing School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut Pan Zhichong College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut Zhu Shihua College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 203-213 0032-0935 242:1<203 2015 242 425 https://doi.org/10.1007/s00425-015-2296-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2296-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ding Wona College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Lin Li School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Botao Department of Botany, La Trobe University, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Xiang Xianbo School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Wu Jing School of Marine Sciences, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Pan Zhichong College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Shihua College of Science and Technology, Ningbo University, 315211, Ningbo, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 203-213 0032-0935 242:1<203 2015 242 425