caa a22 4500 606207554 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2310-8 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2310-8 Identification of a gene controlling variation in the salt tolerance of rapeseed ( Brassica napus L.) [Elektronische Daten] [Hui-Yee Yong, Chunlei Wang, Ian Bancroft, Feng Li, Xiaoming Wu, Hiroyasu Kitashiba, Takeshi Nishio] Main conclusion : By genome-wide association study, QTLs for salt tolerance in rapeseed were detected, and a TSN1 ortholog was identified as a candidate gene responsible for genetic variation in cultivars. Dissecting the genomic regions governing abiotic stress tolerance is necessary for marker-assisted breeding to produce elite breeding lines. In this study, a world-wide collection of rapeseed was evaluated for salt tolerance. These rapeseed accessions showed a large variation for salt tolerance index ranging from 0.311 to 0.999. Although no significant correlation between salt tolerance and Na+ content was observed, there was a significant negative correlation between shoot biomass production under a control condition and salt tolerance. These rapeseed accessions were genotyped by DArTseq for a total of 51,109 genetic markers, which were aligned with ‘pseudomolecules' representative of the genome of rapeseed to locate their hypothetical order for association mapping. A total of 62 QTLs for salt tolerance, shoot biomass, and ion-homeostasis-related traits were identified by association mapping using both the P and Q+K models. Candidate genes located within the QTL regions were also shortlisted. Sequence analysis showed many polymorphisms for BnaaTSN1. Three of them in the coding region resulting in a premature stop codon or frameshift were found in most of the sensitive lines. Loss-of-function mutations showed a significant association with salt tolerance in B. napus. Springer-Verlag Berlin Heidelberg, 2015 Rapeseed nationallicence Salt tolerance nationallicence Salt stress nationallicence Association mapping nationallicence TSN1 nationallicence Yong Hui-Yee Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut Wang Chunlei Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut Bancroft Ian Department of Biology, University of York, Wentworth Way, YO10 5DD, York, UK aut Li Feng Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, China aut Wu Xiaoming Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, China aut Kitashiba Hiroyasu Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut Nishio Takeshi Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 313-326 0032-0935 242:1<313 2015 242 425 https://doi.org/10.1007/s00425-015-2310-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2310-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yong Hui-Yee Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Wang Chunlei Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Bancroft Ian Department of Biology, University of York, Wentworth Way, YO10 5DD, York, UK aut NATIONALLICENCE 700 1- Li Feng Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, China aut NATIONALLICENCE 700 1- Wu Xiaoming Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, China aut NATIONALLICENCE 700 1- Kitashiba Hiroyasu Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Nishio Takeshi Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Miyagi, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 313-326 0032-0935 242:1<313 2015 242 425