caa a22 4500 606207570 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2286-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2286-4 The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes [Elektronische Daten] [Céline Boulard, Michel Bardet, Thierry Chardot, Bertrand Dubreucq, Marina Gromova, Armel Guillermo, Martine Miquel, Nathalie Nesi, Stéphanie Yen-Nicolaÿ, Pascale Jolivet] Main conclusion : The protein, phospholipid and sterol composition of the oil body surface from the seeds of two rapeseed genotypes was compared in order to explain their contrasted oil extractability. In the mature seeds of oleaginous plants, storage lipids accumulate in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids surrounded by a phospholipid monolayer in which structural proteins are embedded. The physical stability of OBs is a consequence of the interactions between proteins and phospholipids. A detailed study of OB characteristics in mature seeds as well as throughout seed development was carried out on two contrasting rapeseed genotypes Amber and Warzanwski. These two accessions were chosen because they differ dramatically in (1) crushing ability, (2) oil extraction yield and, (3) the stability of purified OBs. Warzanwski has higher crushing ability, better oil extraction yield and less stable purified OBs than Amber. OB morphology was investigated in situ using fluorescence microscopy, transmission electron microscopy and pulsed field gradient NMR. During seed development, OB diameter first increased and then decreased 30days after pollination in both Amber and Warzanwski embryos. In mature seeds, Amber OBs were significantly smaller. The protein, phospholipid and sterol composition of the hemi-membrane was compared between the two accessions. Amber OBs were enriched with H-oleosins and steroleosins, suggesting increased coverage of the OB surface consistent with their higher stability. The nature and composition of phospholipids and sterols in Amber OBs suggest that the hemi-membrane would have a more rigid structure than that of Warzanwski OBs. Springer-Verlag Berlin Heidelberg, 2015 Brassica napus nationallicence Hemi-membrane nationallicence Oleosin nationallicence Phospholipid nationallicence Phytosterol nationallicence Steroleosin nationallicence 2D-DIGE : Two-dimensional difference gel electrophoresis nationallicence Dap : Day after pollination nationallicence ER : Endoplasmic reticulum nationallicence FA : Fatty acid nationallicence FAME : Fatty acid methyl ester nationallicence GC : Gas chromatography nationallicence HPTLC : High-performance thin-layer chromatography nationallicence HRMS : High-resolution mass spectrometry nationallicence IEF : Isoelectrofocalization nationallicence OB : Oil body nationallicence MS : Mass spectrometry nationallicence NIRS : Near-infrared spectroscopy nationallicence PA : Phosphatidic acid nationallicence PC : Phosphatidylcholine nationallicence PE : Phosphatidylethanolamine nationallicence PFGNMR : Pulsed field gradient nuclear magnetic resonance nationallicence PG : Phosphatidylglycerol nationallicence PI : Phosphatidylinositol nationallicence PL : Phospholipid nationallicence PS : Phosphatidylserine nationallicence RT : Retention time nationallicence SEM : Standard error of mean nationallicence TAG : Triacylglycerol nationallicence TLC : Thin layer chromatography nationallicence TEM : Transmission electron microscopy nationallicence Boulard Céline INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut Bardet Michel CEA, Service de Chimie Inorganique et Biologique, UMR E3 CEA-Université Joseph Fourier, Grenoble, France aut Chardot Thierry INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut Dubreucq Bertrand INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut Gromova Marina CEA, Service de Chimie Inorganique et Biologique, UMR E3 CEA-Université Joseph Fourier, Grenoble, France aut Guillermo Armel CEA, Structures et Propriétés d'Architectures Moléculaires, UMR 5819 CEA-CNRS-Université Joseph Fourier, Grenoble, France aut Miquel Martine INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut Nesi Nathalie INRA, Institut de Génétique, Environnement et Protection des Plantes, UMR 1349, Le Rheu, France aut Yen-Nicolaÿ Stéphanie Service d'Analyse des Médicaments et Métabolites, IFR IPSIT-Faculté de Pharmacie Université Paris Sud, Châtenay Malabry, France aut Jolivet Pascale INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 53-68 0032-0935 242:1<53 2015 242 425 https://doi.org/10.1007/s00425-015-2286-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2286-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boulard Céline INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut NATIONALLICENCE 700 1- Bardet Michel CEA, Service de Chimie Inorganique et Biologique, UMR E3 CEA-Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Chardot Thierry INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut NATIONALLICENCE 700 1- Dubreucq Bertrand INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut NATIONALLICENCE 700 1- Gromova Marina CEA, Service de Chimie Inorganique et Biologique, UMR E3 CEA-Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Guillermo Armel CEA, Structures et Propriétés d'Architectures Moléculaires, UMR 5819 CEA-CNRS-Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Miquel Martine INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut NATIONALLICENCE 700 1- Nesi Nathalie INRA, Institut de Génétique, Environnement et Protection des Plantes, UMR 1349, Le Rheu, France aut NATIONALLICENCE 700 1- Yen-Nicolaÿ Stéphanie Service d'Analyse des Médicaments et Métabolites, IFR IPSIT-Faculté de Pharmacie Université Paris Sud, Châtenay Malabry, France aut NATIONALLICENCE 700 1- Jolivet Pascale INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 53-68 0032-0935 242:1<53 2015 242 425