caa a22 4500 606207589 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2298-0 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2298-0 Production of deuterated switchgrass by hydroponic cultivation [Elektronische Daten] [Barbara Evans, Garima Bali, Marcus Foston, Arthur Ragauskas, Hugh O'Neill, Riddhi Shah, Joseph McGaughey, David Reeves, Caroline Rempe, Brian Davison] Main conclusion : The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50% D 2 O to obtain biomass with 34% deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls. Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H2O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D2O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by 1H- and 2H-NMR. This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques. Springer-Verlag Berlin Heidelberg, 2015 Biomass nationallicence Switchgrass nationallicence D2O nationallicence Deuteration nationallicence NMR nationallicence FTIR nationallicence Evans Barbara Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Bali Garima Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut Foston Marcus Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut Ragauskas Arthur Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut O'Neill Hugh Biology and Soft Matter Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Shah Riddhi Biology and Soft Matter Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut McGaughey Joseph Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Reeves David Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Rempe Caroline Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Davison Brian Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 215-222 0032-0935 242:1<215 2015 242 425 https://doi.org/10.1007/s00425-015-2298-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2298-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Evans Barbara Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- Bali Garima Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Foston Marcus Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Ragauskas Arthur Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut NATIONALLICENCE 700 1- O'Neill Hugh Biology and Soft Matter Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- Shah Riddhi Biology and Soft Matter Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- McGaughey Joseph Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- Reeves David Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- Rempe Caroline Chemical Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 700 1- Davison Brian Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 215-222 0032-0935 242:1<215 2015 242 425