caa a22 4500 606207600 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2303-7 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2303-7 The complete chloroplast genome of colchicine plants ( Colchicum autumnale L. and Gloriosa superba L.) and its application for identifying the genus [Elektronische Daten] [Pham Nguyen, Jung Kim, Joo-Hwan Kim] Main conclusion : The complete chloroplast genome of two colchicine medicinal plants is reported for the first time. Deletion of ycf 15 gene occurred only in Colchicum but not in Gloriosa and suggests this as a potential marker for delineating the two species. Colchicum autumnale L. and Gloriosa superba L. are well-known sources of colchicine, a type of alkaloid and an ancient anti-inflammatory drug used to prevent gout. Accordingly, this alkaloid has been used as a chemical marker for identifying the expanded Colchicaceae family. In the present study, we report the complete chloroplast genome (cpDNA) sequence of two colchicine medicinal plants (G. superba and C. autumnale) that belong to the tribe Colchiceae of the Colchicaceae family. In C. autumnale, the circular double-stranded cpDNA sequence of 156,462bp consists of two inverted repeat (IR) regions of 27,741bp each, a large single-copy region (LSC) of 84,246bp, and a small single-copy region (SSC) of 16,734bp. The cpDNA sequence of G. superba is longer than that of C. autumnale (157,924bp), which consists of two IRs (28,063bp), an SSC (16,786bp), and an LSC (85,012bp). Significant structural differences between them were observed in the ycf15 gene. ycf15 gene was absent from C. autumnale cpDNA and affected the length of the chloroplast genome between the species. Furthermore, this gene loss event was specific to the expanded genus of Colchicum sensu Vinnersten and Manning. Therefore, this gene may be an effective and powerful molecular marker for identifying the Colchicum genus within the family. Springer-Verlag Berlin Heidelberg, 2015 Chloroplast genome nationallicence Colchicum autumnale nationallicence Gloriosa superba nationallicence ycf 15 nationallicence Gene deletion nationallicence Nguyen Pham Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut Kim Jung Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut Kim Joo-Hwan Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 223-237 0032-0935 242:1<223 2015 242 425 https://doi.org/10.1007/s00425-015-2303-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2303-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nguyen Pham Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Kim Jung Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Kim Joo-Hwan Department of Life Science, Gachon University, Seongnamdaero 1342, 461-701, Seongnam, Gyeonggi-do, Korea aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 223-237 0032-0935 242:1<223 2015 242 425