caa a22 4500 606207619 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2306-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2306-4 Conyza species: distribution and evolution of multiple target-site herbicide resistances [Elektronische Daten] [Maor Matzrafi, Tzipora Lazar, Moshe Sibony, Baruch Rubin] Main conclusion : Distribution of Conyza species is well correlated with human interference. Multiple herbicide resistance is caused by the attempt to overcome resistance to one mode of action by overuse of another. Conyza canadensis (CC) and Conyza bonariensis (CB) are troublesome weeds around the world. Extensive use of herbicides has led to the evolution of numerous Conyza spp. herbicide-resistant populations. Seeds of 91 CC and CB populations were collected across Israel. They were mostly found (86%) in roadsides and urban habitats, two disturbed habitats that had been dramatically impacted by human activities, thus we classify these species as anthropogenic. Although pyrithiobac-sodium was only used in cotton fields, 90% of Conyza spp. populations were identified as pyrithiobac-sodium resistant, suggesting possible natural resistance to pyrithiobac-sodium. CC21 and CC17 C. canadensis populations were highly resistant to all tested ALS inhibitors due to a substitution in the ALS gene from Trp574 to Leu. They were also atrazine resistant due to a substitution in the psbA gene from Ser264 to Gly. The high level of imazapyr and pyrithiobac-sodium resistance observed in the CC10 population was due to an Ala205 to Val substitution. However, high resistance to sulfometuron methyl and pyrithiobac-sodium in population CC6 was due to a point mutation at Pro197 to Ser. All resistant plants of CC21 population showed both psbA (Ser264 to Gly) and ALS (Trp574 to Leu) substitutions, leading us to the conclusion that the attempt to overcome resistance to one mode of action by overuse of another will most likely lead to multiple herbicide resistance. Furthermore, we concluded that only individuals that carry both mutations could survive the shift between the two modes of action and overcome the fitness cost associated with the PSII resistance. Springer-Verlag Berlin Heidelberg, 2015 ALS nationallicence PSII nationallicence Multiple herbicide resistance nationallicence Horseweed nationallicence Hairy fleabane nationallicence CC : Conyza canadensis nationallicence CB : Conyza bonariensis nationallicence ALS : Acetolactate synthase nationallicence PSII : Photosystem II multiple nationallicence MHR : Multiple herbicide resistance nationallicence MOA : Mode of action nationallicence TS : Target site nationallicence DAT : Days after treatment nationallicence ED50 : Effective dose fifty nationallicence Matzrafi Maor The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut Lazar Tzipora The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut Sibony Moshe The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut Rubin Baruch The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 259-267 0032-0935 242:1<259 2015 242 425 https://doi.org/10.1007/s00425-015-2306-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2306-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matzrafi Maor The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut NATIONALLICENCE 700 1- Lazar Tzipora The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut NATIONALLICENCE 700 1- Sibony Moshe The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut NATIONALLICENCE 700 1- Rubin Baruch The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, PO Box 12, 7610001, Rehovot, Israel aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 259-267 0032-0935 242:1<259 2015 242 425