caa a22 4500 606207627 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2305-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2305-5 Small tandem target mimic-mediated blockage of microRNA858 induces anthocyanin accumulation in tomato [Elektronische Daten] [Xiaoyun Jia, Jie Shen, Hui Liu, Fang Li, Na Ding, Changyong Gao, Sitakanta Pattanaik, Barunava Patra, Runzhi Li, Ling Yuan] Main conclusion : Our work strongly suggests that microRNA858 regulates anthocyanin biosynthesis in tomato by modulating the expression of two R2R3 MYB transcription factors, underscoring the importance of microRNAs in the gene regulatory network controlling specialized metabolism in plants. The biological functions of microRNA858 (miR858), a recently identified small RNA, are not well understood. Here, we identified miR858 as a negative regulator of anthocyanin biosynthesis in tomato (Solanum lycopersicum). RNA ligase-mediated 5′RACE cleavage assay showed that miR858 mediates the cleavage of SlMYB7-like and SlMYB48-like transcripts in tomato. Expression analysis revealed an inverse correlation between the accumulation of miR858 and its target SlMYB7-like mRNA, in different tissues of tomato. Subsequently, a small tandem target mimic construct for blocking miR858 (STTM858) was generated and transformed into tomato. The majority of endogenous miR858 was blocked in STTM858 over-expressing tomato plants, whereas SlMYB7-like transcripts increased significantly. Concomitantly, upregulated expression was detected for several anthocyanin biosynthetic genes, including PAL, CHS, DFR, ANS and 3GT. As a result, anthocyanins were highly accumulated in young seedlings, leaves, stems and leaf buds of the transgenic plants under normal growth conditions. In addition, over-expression of STTM858 in tomato activated another MYB transcription factor, SlMYB48, implicating the possible involvement of SlMYB48 in anthocyanin biosynthesis. Springer-Verlag Berlin Heidelberg, 2015 Tomato nationallicence MiR858 nationallicence SlMYB7 - like nationallicence SlMYB48 - like nationallicence Anthocyanin biosynthesis nationallicence ANS : Anthocyanidin synthase nationallicence CHS : Chalcone synthase nationallicence DFR : Dihydroflavonol reductase nationallicence miRNA : MicroRNA nationallicence PAL : Phenylalanine ammonialyase nationallicence pri-miRNA : Primary miRNA nationallicence qRT-PCR : Quantitative real-time reverse transcription PCR nationallicence STTM : Small tandem target mimic nationallicence 3GT : Flavonol-3-glucosyltransferase nationallicence Jia Xiaoyun College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Shen Jie College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Liu Hui College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Li Fang College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Ding Na College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Gao Changyong College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Pattanaik Sitakanta Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky, 40546, Lexington, KY, USA aut Patra Barunava Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky, 40546, Lexington, KY, USA aut Li Runzhi College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Yuan Ling College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 283-293 0032-0935 242:1<283 2015 242 425 https://doi.org/10.1007/s00425-015-2305-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2305-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Xiaoyun College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Shen Jie College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Liu Hui College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Li Fang College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Ding Na College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Gao Changyong College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Pattanaik Sitakanta Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky, 40546, Lexington, KY, USA aut NATIONALLICENCE 700 1- Patra Barunava Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky, 40546, Lexington, KY, USA aut NATIONALLICENCE 700 1- Li Runzhi College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 700 1- Yuan Ling College of Life Science, Shanxi Agricultural University, 030801, Taigu, Shanxi, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 283-293 0032-0935 242:1<283 2015 242 425