caa a22 4500 606207635 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2287-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2287-3 Association mapping in Populus reveals the interaction between Pto - miR530a and its target Pto - KNAT1 [Elektronische Daten] [Xiaohui Yang, Qingzhang Du, Jinhui Chen, Bowen Wang, Deqiang Zhang] Main conclusion : We used transcript profiling and multi-SNP association to investigate the genetic regulatory relationship between miRNA Pto-miR530a and its target Pto-KNAT1, identifying additive, dominant, and epistatic effects. MicroRNAs (miRNAs) play crucial roles in the post-transcriptional regulation of plant growth and development; indeed, many studies have described the importance of miRNA-target interactions in herbaceous species. However, elucidation of the miRNA-target interactions in trees may require novel strategies. In the present study, we describe a strategy combining expression profiling by reverse transcription quantitative PCR (RT-qPCR) and association mapping with multiple single nucleotide polymorphisms (SNPs) to evaluate the interaction between Pto-miR530a and its target Pto-KNAT1 in Populus tomentosa. RT-qPCR analysis showed a negative correlation (r=−0.62, P<0.05) between expression levels of Pto-miR530a and Pto-KNAT1 in eight tissues. We used a Bayesian hierarchical model to identify allelic variants of Pto-miR530a and Pto-KNAT1 that associated with eight traits related to growth and wood properties, in a population of 460 unrelated individuals of P. tomentosa. This analysis identified 27 associations, with the proportions of phenotypic variance (R 2) contributed by each SNP ranging of 0.82-15.81%, the additive effects of each SNP ranging of 0.16-18.09, and the dominant effects ranging from −14.09 to 19.00. Epistatic interaction models showed a strong interaction among SNPs in the miRNA target with R 2 of 0.1-3.56%, and information gain of significant SNP pairs of −3.09 to 0.93%, representing the regulatory interactions between the miRNA and the mRNA. Thus, we used a new strategy that combines association genetics and expression profiling based on SNPs to study the regulatory relationship between this miRNA and its target mRNA, thereby providing novel advances in our understanding of the genetic architecture of important traits. Springer-Verlag Berlin Heidelberg, 2015 Association genetics nationallicence Epistasis nationallicence MiRNA/target interaction nationallicence Transcript profiling nationallicence Tree growth nationallicence Wood properties nationallicence 5′-UTR : 5′-untranslated region nationallicence DBH : Diameter at breast height nationallicence FL : Fiber length nationallicence FW : Fiber width nationallicence fGWAS : Functional genome-wide association studies nationallicence HC : Holocellulose content nationallicence IG : Information gain nationallicence LC : Lignin content nationallicence LD : Linkage disequilibrium nationallicence MFA : Microfiber angle nationallicence miRNAs : MicroRNAs nationallicence MDR3.0.2 : Multifactor dimensionality reduction 3.0.2 nationallicence PCN : POPCORONA nationallicence PCA : Principle component analysis nationallicence RT-qPCR : Reverse transcription quantitative PCR nationallicence SNP : Single nucleotide polymorphism nationallicence V : Stem volume nationallicence H : Tree height nationallicence Yang Xiaohui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Chen Jinhui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Wang Bowen National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 77-95 0032-0935 242:1<77 2015 242 425 https://doi.org/10.1007/s00425-015-2287-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2287-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xiaohui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jinhui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Bowen National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 77-95 0032-0935 242:1<77 2015 242 425