caa a22 4500 606207643 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2304-6 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2304-6 Next-generation sequencing (NGS) transcriptomes reveal association of multiple genes and pathways contributing to secondary metabolites accumulation in tuberous roots of Aconitum heterophyllum Wall [Elektronische Daten] [Tarun Pal, Nikhil Malhotra, Sree Chanumolu, Rajinder Chauhan] Main conclusion : The transcriptomes of Aconitum heterophyllum were assembled and characterized for the first time to decipher molecular components contributing to biosynthesis and accumulation of metabolites in tuberous roots. Aconitum heterophyllum Wall., popularly known as Atis, is a high-value medicinal herb of North-Western Himalayas. No information exists as of today on genetic factors contributing to the biosynthesis of secondary metabolites accumulating in tuberous roots, thereby, limiting genetic interventions towards genetic improvement of A. heterophyllum. Illumina paired-end sequencing followed by de novo assembly yielded 75,548 transcripts for root transcriptome and 39,100 transcripts for shoot transcriptome with minimum length of 200bp. Biological role analysis of root versus shoot transcriptomes assigned 27,596 and 16,604 root transcripts; 12,340 and 9398 shoot transcripts into gene ontology and clusters of orthologous group, respectively. KEGG pathway mapping assigned 37 and 31 transcripts onto starch-sucrose metabolism while 329 and 341 KEGG orthologies associated with transcripts were found to be involved in biosynthesis of various secondary metabolites for root and shoot transcriptomes, respectively. In silico expression profiling of the mevalonate/2-C-methyl-d-erythritol 4-phosphate (non-mevalonate) pathway genes for aconites biosynthesis revealed 4 genes HMGR (3-hydroxy-3-methylglutaryl-CoA reductase), MVK (mevalonate kinase), MVDD (mevalonate diphosphate decarboxylase) and HDS (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase) with higher expression in root transcriptome compared to shoot transcriptome suggesting their key role in biosynthesis of aconite alkaloids. Five genes, GMPase (geranyl diphosphate mannose pyrophosphorylase), SHAGGY, RBX1 (RING-box protein 1), SRF receptor kinases and β-amylase, implicated in tuberous root formation in other plant species showed higher levels of expression in tuberous roots compared to shoots. A total of 15,487 transcription factors belonging to bHLH, MYB, bZIP families and 399 ABC transporters which regulate biosynthesis and accumulation of bioactive compounds were identified in root and shoot transcriptomes. The expression of 5 ABC transporters involved in tuberous root development was validated by quantitative PCR analysis. Network connectivity diagrams were drawn for starch-sucrose metabolism and isoquinoline alkaloid biosynthesis associated with tuberous root growth and secondary metabolism, respectively, in root transcriptome of A. heterophyllum. The current endeavor will be of practical importance in planning a suitable genetic intervention strategy for the improvement of A. heterophyllum. Springer-Verlag Berlin Heidelberg, 2015 Aconitum nationallicence Network connectivity diagrams nationallicence Pathway mapping nationallicence RNA-seq nationallicence Transcript abundance nationallicence Transcriptome analysis nationallicence Tuberous roots nationallicence AHSR : Root transcriptome nationallicence AHSS : Shoot transcriptome nationallicence COG : Cluster of orthologous group nationallicence GO : Gene ontology nationallicence KO : KEGG orthology nationallicence MEP : 2-C-Methyl-d-erythritol 4-phosphate nationallicence MVA : Mevalonate nationallicence NGS : Next-generation sequencing nationallicence FPKM : Fragments per kilobase of transcript per million fragments mapped nationallicence SSR : Simple sequence repeat nationallicence Pal Tarun Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut Malhotra Nikhil Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut Chanumolu Sree Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut Chauhan Rajinder Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 239-258 0032-0935 242:1<239 2015 242 425 https://doi.org/10.1007/s00425-015-2304-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2304-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pal Tarun Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Malhotra Nikhil Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Chanumolu Sree Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Chauhan Rajinder Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, 173234, Waknaghat, Himachal Pradesh, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 239-258 0032-0935 242:1<239 2015 242 425