caa a22 4500 60620766X CHVBK 20210128101018.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2297-1 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2297-1 Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns [Elektronische Daten] [Zhong Wang, Pan Wei, Mingzhu Wu, Yalong Xu, Feng Li, Zhaopeng Luo, Jianfeng Zhang, Ang Chen, Xiaodong Xie, Peijian Cao, Fucheng Lin, Jun Yang] Main conclusion : Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum, N. sylvestris and N. tomentosiformis, respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1-4, Sus5, and Sus6-7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1/2. Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses. The Author(s), 2015 Phylogeny nationallicence Stress nationallicence Sucrose nationallicence Tobacco nationallicence Sus : Sucrose synthase nationallicence Inv : Invertase nationallicence UDP : Uridine diphosphate nationallicence PEG : Polyethylene glycol nationallicence gDNA : Genomic DNA nationallicence CDS : Coding sequence nationallicence Mw : Molecular weight nationallicence pI : Isoelectric point nationallicence Wang Zhong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Wei Pan China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Wu Mingzhu China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Xu Yalong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Li Feng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Luo Zhaopeng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Zhang Jianfeng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Chen Ang China School of Life Sciences, Xiamen University, 361102, Xiamen, Fujian, China aut Xie Xiaodong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Cao Peijian China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Lin Fucheng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Yang Jun China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 153-166 0032-0935 242:1<153 2015 242 425 https://doi.org/10.1007/s00425-015-2297-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2297-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Wei Pan China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Wu Mingzhu China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Xu Yalong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Li Feng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Luo Zhaopeng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Zhang Jianfeng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Chen Ang China School of Life Sciences, Xiamen University, 361102, Xiamen, Fujian, China aut NATIONALLICENCE 700 1- Xie Xiaodong China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Cao Peijian China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Lin Fucheng China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 700 1- Yang Jun China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, 450001, Zhengzhou, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 153-166 0032-0935 242:1<153 2015 242 425