caa a22 4500 606207686 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2301-9 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2301-9 Symbiotic in vitro seed propagation of Dendrobium [Elektronische Daten] fungal and bacterial partners and their influence on plant growth and development [Jaime Teixeira da Silva, Elena Tsavkelova, Songjun Zeng, Tzi Ng, S. Parthibhan, Judit Dobránszki, Jean Cardoso, M. Rao] The genus Dendrobium is one of the largest genera of the Orchidaceae Juss. family, although some of its members are the most threatened today. The reason why many species face a vulnerable or endangered status is primarily because of anthropogenic interference in natural habitats and commercial overexploitation. The development and application of modern techniques and strategies directed towards in vitro propagation of orchids not only increases their number but also provides a viable means to conserve plants in an artificial environment, both in vitro and ex vitro, thus providing material for reintroduction. Dendrobium seed germination and propagation are challenging processes in vivo and in vitro, especially when the extreme specialization of these plants is considered: (1) their biotic relationships with pollinators and mycorrhizae; (2) adaptation to epiphytic or lithophytic life-styles; (3) fine-scale requirements for an optimal combination of nutrients, light, temperature, and pH. This review also aims to summarize the available data on symbiotic in vitro Dendrobium seed germination. The influence of abiotic factors as well as composition and amounts of different exogenous nutrient substances is examined. With a view to better understanding how to optimize and control in vitro symbiotic associations, a part of the review describes the strong biotic relations of Dendrobium with different associative microorganisms that form microbial communities with adult plants, and also influence symbiotic seed germination. The beneficial role of plant growth-promoting bacteria is also discussed. Springer-Verlag Berlin Heidelberg, 2015 Dendrobium germination and propagation nationallicence Mycorrhiza nationallicence Orchid-associated bacteria nationallicence Orchidaceae nationallicence Symbiotic seed germination nationallicence ABA : Abscisic acid nationallicence AMF : Arbuscular mycorrhizal fungi nationallicence C : Carbon nationallicence CMA : Cornmeal agar nationallicence DW : Dry weight nationallicence FW : Fresh weight nationallicence GA : Gibberellin nationallicence GA3 : Gibberellic acid nationallicence IAA : Indole-3-acetic acid nationallicence ITS : Internal transcribed spacer nationallicence MH : Mycoheterotrophy nationallicence N : Nitrogen nationallicence OMA : Oatmeal agar nationallicence OMF : Orchid mycorrhizal fungi nationallicence PGPR : Plant growth-promoting rhizobacteria nationallicence PGR : Plant growth regulator nationallicence PLB : Protocorm-like body nationallicence SEM : Scanning electron microscopy nationallicence Trp : Tryptophan nationallicence Teixeira da Silva Jaime P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, 761-0799, Kagawa-Ken, Japan aut Tsavkelova Elena Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Leninskie gory 1-12, 119234, Moscow, Russia aut Zeng Songjun Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China aut Parthibhan S. Department of Plant Science, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut Dobránszki Judit Research Institute of Nyíregyháza, University of Debrecen, P.O. Box 12, 4400, Nyíregyháza, Hungary aut Cardoso Jean Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, CEP 13.600-970, Araras, Brazil aut Rao M. Department of Plant Science, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 1-22 0032-0935 242:1<1 2015 242 425 https://doi.org/10.1007/s00425-015-2301-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2301-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Teixeira da Silva Jaime P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, 761-0799, Kagawa-Ken, Japan aut NATIONALLICENCE 700 1- Tsavkelova Elena Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Leninskie gory 1-12, 119234, Moscow, Russia aut NATIONALLICENCE 700 1- Zeng Songjun Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 700 1- Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Parthibhan S. Department of Plant Science, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut NATIONALLICENCE 700 1- Dobránszki Judit Research Institute of Nyíregyháza, University of Debrecen, P.O. Box 12, 4400, Nyíregyháza, Hungary aut NATIONALLICENCE 700 1- Cardoso Jean Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, CEP 13.600-970, Araras, Brazil aut NATIONALLICENCE 700 1- Rao M. Department of Plant Science, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 1-22 0032-0935 242:1<1 2015 242 425