caa a22 4500 606207694 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2307-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2307-3 Transcript abundance patterns of Populus C - repeat binding factor2 orthologs and genetic association of PsCBF2 allelic variation with physiological and biochemical traits in response to abiotic stress [Elektronische Daten] [Ying Li, Baohua Xu, Qingzhang Du, Deqiang Zhang] Main conclusion : We conducted a candidate gene-based approach to search for genetic associations between 10 SNPs in PsCBF2 and 10 abiotic stress-related traits. The increasing incidence of abiotic stresses and the limitations of available treatments, particularly in trees, highlight the need to improve our understanding of the mechanisms of stress responses. In Arabidopsis, C-repeat binding factor 2 (CBF2) plays an important role in freezing tolerance and cold acclimation. Here, we isolated orthologs of CBF2 from five Populus species. Expression profiling revealed that the Populus CBF2s were preferentially induced in response to cold, with CBF2 transcript abundances ranging from 5.4- to 62-fold higher than in unstressed controls of the corresponding species. In addition, we used a candidate gene-based approach in Populus simonii Carr. to identify single nucleotide polymorphisms (SNPs) in PsCBF2 associated with physiological and biochemical traits. PsCBF2 showed high nucleotide diversity (π T=0.00549, θ w=0.01406) and low average linkage disequilibrium (r 2=0.061). Association studies in 528 individuals of an association population showed that nine SNPs (false discovery rate Q<0.10) and one haplotype (Q<0.10) were significantly associated with differences in four physiological and biochemical traits (P<0.005), with each marker explaining 1.31-5.87% of the total variance in the corresponding trait. PsCBF2 transcript levels differed significantly in abundance among genotypic classes for most of the significant SNPs. Identification of these significant associations will help reveal the molecular basis of physiological differences and provide a starting point for marker-assisted selection for traits involved in stress tolerance in P. simonii. Springer-Verlag Berlin Heidelberg, 2015 Abiotic stress nationallicence Association studies nationallicence DREB1/CBF-type transcription factor nationallicence Poplar nationallicence Species-specific transcript abundance pattern nationallicence ABA : Abscisic acid nationallicence AP2/ERF : APETALA2/ethylene-responsive element binding factor (ERF) nationallicence CAT : Catalase nationallicence CBF : C-repeat binding factor nationallicence DRE/CRT : Dehydration-responsive element and C-repeat motif nationallicence DREBs : DRE binding proteins nationallicence LD : Linkage disequilibrium nationallicence MAS : Marker-assisted selection nationallicence ORF : Open reading frame nationallicence QTLs : Quantitative trait loci nationallicence ROS : Reactive oxygen species nationallicence SNPs : Single nucleotide polymorphisms nationallicence TFs : Transcription factors nationallicence Li Ying National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Xu Baohua National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 295-312 0032-0935 242:1<295 2015 242 425 https://doi.org/10.1007/s00425-015-2307-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2307-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ying National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xu Baohua National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 295-312 0032-0935 242:1<295 2015 242 425