caa a22 4500 606207708 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2288-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2288-2 Functional co-expression of a fungal ferulic acid esterase and a β-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction [Elektronische Daten] [Marcia Buanafina, Sue Dalton, Tim Langdon, E. Timms-Taravella, Erica Shearer, Phillip Morris] Main conclusion : Improved post-harvest cell wall deconstruction of tall fescue leaves has been demonstrated by in-planta co-expression of a constitutively expressed ferulic acid esterase together with a senescence-induced β-1,4 endoxylanase. Tall fescue plants (Festuca arundinacea) constitutively expressing vacuole- or apoplast-targeted ferulic acid esterase from Aspergillus niger were retransformed with a senescence-induced and apoplast-targeted β-1,4 endo-xylanase from Trichoderma reesei. Enzyme activities in co-expressing plants stabilized after repeated vegetative propagation, with xylanase activity in senescent leaves increasing and ferulic acid esterase activity decreasing after tillering. Plants co-expressing both enzymes in the apoplast, with the lowest levels of ferulate monomers and dimers and the lowest levels of cell wall arabinoxylans, released ten times more cell wall hydroxycinnamic acids and five times more arabinoxylan from the cell wall on autodigestion compared to expression of ferulic acid esterase or xylanase alone. These plants also showed a 31% increase in cellulase-mediated release of reducing sugars, a 5% point increase in in vitro dry matter digestibility and a 23% increase in acetyl bromide-soluble lignin. However, plant growth was adversely affected by expressing FAE in the apoplast, giving plants with narrower shorted leaves, and a 71% decrease in biomass. Springer-Verlag Berlin Heidelberg, 2015 Ferulic acid esterase nationallicence Xylanase nationallicence Cell wall structure nationallicence Digestibility nationallicence Ferulates nationallicence AIR : Alcohol insoluble residues nationallicence AX : Arabinoxylan nationallicence XYN2 : β-1,4 endo-xylanase nationallicence FA : Ferulic acid nationallicence FAEA : Ferulic acid esterase nationallicence HCAs : Hydroxycinnamic acids nationallicence HPAEC : Anion exchange chromatography nationallicence HPLC : High performance liquid chromatography nationallicence IVDMD : In vitro dry matter digestibility nationallicence LmSee1 : Lolium multiflorum senescence promoter nationallicence PAHBAH : p-Hydroxybenzoic acid hydrazide nationallicence p CA : p-Coumaric acid nationallicence TFA : Trifluoroacetic acid nationallicence Buanafina Marcia Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, 16802, University Park, PA, USA aut Dalton Sue Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut Langdon Tim Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut Timms-Taravella E. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut Shearer Erica Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, 16802, University Park, PA, USA aut Morris Phillip Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 97-111 0032-0935 242:1<97 2015 242 425 https://doi.org/10.1007/s00425-015-2288-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2288-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buanafina Marcia Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Dalton Sue Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut NATIONALLICENCE 700 1- Langdon Tim Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut NATIONALLICENCE 700 1- Timms-Taravella E. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut NATIONALLICENCE 700 1- Shearer Erica Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Morris Phillip Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Wales, UK aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 97-111 0032-0935 242:1<97 2015 242 425