caa a22 4500 606207724 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2385-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2385-2 Accumulation of mannitol in the cytoplasm and vacuole during the expansion of sepal cells associated with flower opening in Delphinium × belladonna cv. Bellamosum [Elektronische Daten] [Ryo Norikoshi, Kunio Yamada, Tomoko Niki, Kazuo Ichimura] Main conclusion : The role of mannitol differs from that of glucose, fructose and sucrose in sepal cell expansion associated with flower opening in Delphinium×belladonna. Sepals of Delphinium×belladonna are colored and much larger than the petals. To determine whether the role of mannitol in sepal growth associated with flower opening differs from those of ubiquitous metabolic sugars including glucose, fructose and sucrose, we investigated changes in cell number, subcellular concentrations of soluble carbohydrates, and osmotic potential in sepals during flower opening in Delphinium×belladonna cv. Bellamosum. The number of epidermal cells in the sepals did not increase from the stage when sepal pigmentation started, whereas the cell area increased during flower opening, indicating that petal growth during flower opening depends on cell expansion. Mannitol concentrations in the vacuole at three different stages were approximately 100mM, which were much higher than the other carbohydrate concentrations, but they decreased slightly at open stage. In contrast, mannitol concentration in the cytoplasm was 56mM at bud stage, but it increased to 104mM at open stage. Glucose and fructose concentrations in the vacuole at open stage increased to 45 and 56mM, respectively. Total osmotic potential in apoplast and symplast, which was partially due to soluble carbohydrates, was almost constant during flower opening. Therefore, mannitol may be acting constitutively as the main osmoticum in the vacuole where it may contribute to the maintenance of the osmotic balance between the cytoplasm and vacuole in open flowers. The role of mannitol differs from those of glucose, fructose, and sucrose in sepal cell expansion in Delphinium×belladonna. Springer-Verlag Berlin Heidelberg, 2015 Cell division nationallicence Cell expansion nationallicence Mannitol nationallicence Sepal growth nationallicence Vacuole nationallicence DW : Dry weight nationallicence FW : Fresh weight nationallicence HPLC : High-performance liquid chromatography nationallicence Norikoshi Ryo NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut Yamada Kunio NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut Niki Tomoko NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut Ichimura Kazuo NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1467-1477 0032-0935 242:6<1467 2015 242 425 https://doi.org/10.1007/s00425-015-2385-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2385-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Norikoshi Ryo NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Yamada Kunio NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Niki Tomoko NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ichimura Kazuo NARO Institute of Floricultural Science, Fujimoto, 305-8519, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1467-1477 0032-0935 242:6<1467 2015 242 425