caa a22 4500 606207759 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2375-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2375-4 Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls [Elektronische Daten] [Valérie Cornuault, Fanny Buffetto, Maja Rydahl, Susan Marcus, Thomas Torode, Jie Xue, Marie-Jeanne Crépeau, Nuno Faria-Blanc, William Willats, Paul Dupree, Marie-Christine Ralet, J. Knox] Main conclusion : The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links. The Author(s), 2015 Arabinogalactan-proteins nationallicence Cell wall nationallicence Glucuronoxylan nationallicence Pectin nationallicence Polysaccharides nationallicence Rhamnogalacturonan-I nationallicence AGP : Arabinogalactan-protein nationallicence EDC : Epitope detection chromatography nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence GalA : Galacturonic acid nationallicence GAX : Glucuronoarabinoxylan nationallicence GlcA : Glucuronic acid nationallicence GH : Glycosyl hydrolase nationallicence HG : Homogalacturonan nationallicence HRP : Horseradish peroxidase nationallicence MAbs : Monoclonal antibodies nationallicence PBS : Phosphate-buffered saline nationallicence RG-I : Rhamnogalacturonan-I nationallicence Cornuault Valérie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Buffetto Fanny UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut Rydahl Maja Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark aut Marcus Susan Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Torode Thomas Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Xue Jie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Crépeau Marie-Jeanne UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut Faria-Blanc Nuno Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK aut Willats William Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark aut Dupree Paul Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK aut Ralet Marie-Christine UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut Knox J. Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1321-1334 0032-0935 242:6<1321 2015 242 425 https://doi.org/10.1007/s00425-015-2375-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2375-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cornuault Valérie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Buffetto Fanny UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut NATIONALLICENCE 700 1- Rydahl Maja Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark aut NATIONALLICENCE 700 1- Marcus Susan Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Torode Thomas Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Xue Jie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Crépeau Marie-Jeanne UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut NATIONALLICENCE 700 1- Faria-Blanc Nuno Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK aut NATIONALLICENCE 700 1- Willats William Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark aut NATIONALLICENCE 700 1- Dupree Paul Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK aut NATIONALLICENCE 700 1- Ralet Marie-Christine UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France aut NATIONALLICENCE 700 1- Knox J. Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1321-1334 0032-0935 242:6<1321 2015 242 425