caa a22 4500 606207767 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2374-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2374-5 Quantitative proteomics analysis reveals that S -nitrosoglutathione reductase (GSNOR) and nitric oxide signaling enhance poplar defense against chilling stress [Elektronische Daten] [Tielong Cheng, Jinhui Chen, Abd_Allah EF, Pengkai Wang, Guangping Wang, Xiangyang Hu, Jisen Shi] Main conclusion : NO acts as the essential signal to enhance poplar tolerance to chilling stress via antioxidant enzyme activities and protein S -nitrosylation modification, NO signal is also strictly controlled by S -nitrosoglutathione reductase and nitrate reductase to avoid the over-accumulation of reactive nitrogen species. Poplar (Populus trichocarpa) are fast growing woody plants with both ecological and economic value; however, the mechanisms by which poplar adapts to environmental stress are poorly understood. In this study, we used isobaric tags for relative and absolute quantification proteomic approach to characterize the response of poplar exposed to cold stress. We identified 114 proteins that were differentially expressed in plants exposed to cold stress. In particular, some of the proteins are involved in reactive oxygen species (ROS) and reactive nitrogen species (RNS) metabolism. Further physiological analysis showed that nitric oxide (NO) signaling activated a series of downstream defense responses. We further demonstrated that NO activated antioxidant enzyme activities and S-nitrosoglutathione reductase (GSNOR) activities, which would reduce ROS and RNS toxicity and thereby enhance poplar tolerance to cold stress. Suppressing NO accumulation or GSNOR activity aggravated cold damage to poplar leaves. Moreover, our results showed that RNS can suppress the activities of GSNOR and NO nitrate reductase (NR) by S-nitrosylation to fine-tune the NO signal and modulate ROS levels by modulating the S-nitrosylation of ascorbate peroxidase protein. Hence, our data demonstrate that NO signaling activates multiple pathways that enhance poplar tolerances to cold stress, and that NO signaling is strictly controlled through protein post-translational modification by S-nitrosylation. Springer-Verlag Berlin Heidelberg, 2015 Cold stress nationallicence Nitric oxide nationallicence Poplar nationallicence Proteome nationallicence S -Nitrosoglutathione reductase nationallicence ABA : Abscisic acid nationallicence APX : Ascorbate peroxidase nationallicence cPTIO : 2-(4-Carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide nationallicence DA : Dodecanoic acid nationallicence DHAR : Dehydroascorbate reductase nationallicence GR : Glutathione reductase nationallicence GSNO : S-nitrosoglutathione nationallicence GSNOR : GSNO reductase nationallicence MDA : Malondialdehyde nationallicence NO : Nitric oxide nationallicence NOS : NO synthase nationallicence NR : Nitrate reductase nationallicence JA : Jasmonic acid nationallicence MDHAR : Monodehydroascorbate reductase nationallicence RNS : Reactive nitrogen species nationallicence ROS : Reactive oxygen species nationallicence SNO : S-nitrosothiol nationallicence Cheng Tielong Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut Chen Jinhui Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut EF Abd_Allah Department of Plant Production, Faculty of Food & Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia aut Wang Pengkai Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut Wang Guangping Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut Hu Xiangyang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, China aut Shi Jisen Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1361-1390 0032-0935 242:6<1361 2015 242 425 https://doi.org/10.1007/s00425-015-2374-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2374-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Tielong Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Chen Jinhui Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- EF Abd_Allah Department of Plant Production, Faculty of Food & Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Wang Pengkai Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Wang Guangping Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Hu Xiangyang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, China aut NATIONALLICENCE 700 1- Shi Jisen Key Laboratory of Forest Genetics & Biogeography, Ministry of Education, Nanjing Forest University, 210037, Nanjing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1361-1390 0032-0935 242:6<1361 2015 242 425