caa a22 4500 606207775 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2390-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2390-5 Overexpression of a phospholipase Dα gene from Ammopiptanthus nanus enhances salt tolerance of phospholipase Dα1-deficient Arabidopsis mutant [Elektronische Daten] [Hao Yu, Tai Yong, Hong Li, Yan Liu, Shu Zhou, Feng Fu, Wan Li] Main conclusion : A phospholipase Dα gene ( AnPLDα ) was cloned from xerophytic desert plant Ammopiptanthus nanus and its overexpression enhanced salt tolerance of a PLDα1 deficient Arabidopsis mutant. Phospholipase Dα (PLDα) hydrolyzes phosphatidylcholine to produce phosphatidic acid, and plays crucial role in plant tolerance to abiotic stress. In this study, a phospholipase Dα gene (AnPLDα) was cloned from xerophyte Ammopiptanthus nanus by the methods of homologous cloning and rapid amplification of cDNA ends, and evaluated for its function in stress tolerance. The full-length cDNA was 2832bp long, containing an open reading frame of 2427bp that encodes 808 amino acids. The putative protein was predicted to be localized to the cytoplasm and this was confirmed by transient expression of a fluorescent fusion protein. The endogenous expression of the AnPLDα gene was induced by high salt, dehydration, cold and abscisic acid. The heterologous expression of the AnPLDα gene improved salt tolerance of an Arabidopsis pldα1 knocked out mutant, and positively regulated the expression of the AtABI, AtNCED, AtRD29A, AtRD29B and AtADH genes. Therefore, the AnPLDα gene was concluded to be involved in response to abiotic stress. The AnPLDα gene is a hopeful candidate for transgenic application to improve stress tolerance of commercial crops. Springer-Verlag Berlin Heidelberg, 2015 Abiotic stress nationallicence Crops nationallicence Gene cloning nationallicence Phospholipase nationallicence Stress tolerance nationallicence ABA : Abscisic acid nationallicence eGFP : Enhanced green fluorescent protein nationallicence ORF : Open reading frame nationallicence PA : Phosphatidic acid nationallicence PLDα : Phospholipase Dα nationallicence Yu Hao Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Yong Tai Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Li Hong Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Liu Yan Faculty of Plant Science, Tarim University, 843300, Alar, Xinjiang, People's Republic of China aut Zhou Shu Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Fu Feng Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Li Wan Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1495-1509 0032-0935 242:6<1495 2015 242 425 https://doi.org/10.1007/s00425-015-2390-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2390-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Hao Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Yong Tai Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Li Hong Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yan Faculty of Plant Science, Tarim University, 843300, Alar, Xinjiang, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Shu Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Fu Feng Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Li Wan Maize Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1495-1509 0032-0935 242:6<1495 2015 242 425