caa a22 4500 606207783 CHVBK 20210128101018.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2392-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2392-3 The short-term response of Arabidopsis thaliana (C3) and Zea mays (C4) chloroplasts to red and far red light [Elektronische Daten] [Maksymilian Zienkiewicz, Anna Drożak, Wioleta Wasilewska, Ilona Bacławska, Ewa Przedpełska-Wąsowicz, Elżbieta Romanowska] Main conclusion : Light quality has various effects on photochemistry and protein phosphorylation in Zea mays and Arabidopsis thaliana thylakoids due to different degrees of light penetration across leaves and redox status in chloroplasts. The effect of the spectral quality of light (red, R and far red, FR) on the function of thylakoid proteins in Zea mays and Arabidopsis thaliana was investigated. It was concluded that red light stimulates PSII activity in A. thaliana thylakoids and in maize bundle sheath (BS) thylakoids, but not in mesophyll (M) thylakoids. The light quality did not change PSI activity in M thylakoids of maize. FR used after a white light period increased PSI activity significantly in maize BS and only slightly in A. thaliana thylakoids. As shown by blue native (BN)-PAGE followed by SDS-PAGE, proteins were differently phosphorylated in the thylakoids, indicating their different functions. FR light increased dephosphorylation of LHCII proteins in A. thaliana thylakoids, whereas in maize, dephosphorylation did not occur at all. The rate of phosphorylation was higher in maize BS than in M thylakoids. D1 protein phosphorylation increased in maize and decreased in A. thaliana upon irradiation with both R and growth light (white light, W). Light variations did not change the level of proteins in thylakoids. Our data strongly suggest that response to light quality is a species-dependent phenomenon. We concluded that the maize chloroplasts were differently stimulated, probably due to different degrees of light penetration across the leaf and thereby the redox status in the chloroplasts. These acclimation changes induced by light quality are important in the regulation of chloroplast membrane flexibility and thus its function. Springer-Verlag Berlin Heidelberg, 2015 Acclimation to light quality nationallicence Bundle sheath chloroplasts nationallicence Mesophyll chloroplasts nationallicence PSI nationallicence PSII nationallicence Protein phosphorylation nationallicence Red and far red light nationallicence Thylakoids nationallicence BS : Bundle sheath nationallicence BN-PAGE : Blue native electrophoresis nationallicence CET : Cyclic electron transport nationallicence FR : Far red light nationallicence LHCII : Chlorophyll a/b- binding protein of photosystem II nationallicence M : Mesophyll nationallicence R : Red light nationallicence W : White light nationallicence Zienkiewicz Maksymilian Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Drożak Anna Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Wasilewska Wioleta Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Bacławska Ilona Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Przedpełska-Wąsowicz Ewa Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Romanowska Elżbieta Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1479-1493 0032-0935 242:6<1479 2015 242 425 https://doi.org/10.1007/s00425-015-2392-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2392-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zienkiewicz Maksymilian Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Drożak Anna Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Wasilewska Wioleta Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Bacławska Ilona Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Przedpełska-Wąsowicz Ewa Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Romanowska Elżbieta Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1479-1493 0032-0935 242:6<1479 2015 242 425