caa a22 4500 606207805 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2382-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2382-5 Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria [Elektronische Daten] [Amel Chamam, Florence Wisniewski-Dyé, Gilles Comte, Cédric Bertrand, Claire Prigent-Combaret] Main conclusion : Profiling of plant secondary metabolite allows to differentiate the different types of ecological interactions established between rice and bacteria. Rice responds to ecologically distinct bacteria by altering its content of flavonoids and hydroxycinnamic acid derivatives. Plants' growth and physiology are strongly influenced by the biotic interactions that plants establish with soil bacterial populations. Plants are able to sense and to respond accordingly to ecologically distinct bacteria, by inducing defense pathways against pathogens to prevent parasitic interactions, and by stimulating the growth of root-associated beneficial or commensal bacteria through root exudation. Plant secondary metabolism is expected to play a major role in this control. However, secondary metabolite responses of a same plant to cooperative, commensal and deleterious bacteria have so far never been compared. The impact of the plant growth-promoting rhizobacteria (PGPR) Azospirillum lipoferum 4B on the secondary metabolite profiles of two Oryza sativa L. cultivars (Cigalon and Nipponbare) was compared to that of a rice pathogen Burkholderia glumae AU6208, the causing agent of bacterial panicle blight and of a commensal environmental bacteria Escherichia coli B6. Root and shoot rice extracts were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Principal component analyses (PCAs) pinpointed discriminant secondary metabolites, which were characterized by mass spectrometry. Direct comparison of metabolic profiles evidenced that each bacterial ecological interaction induced distinct qualitative and quantitative modifications of rice secondary metabolism, by altering the content of numerous flavonoid compounds and hydroxycinnamic acid (HCA) derivatives. Secondary metabolism varied according to the cultivars and the interaction types, demonstrating the relevance of secondary metabolic profiling for studying plant-bacteria biotic interactions. Springer-Verlag Berlin Heidelberg, 2015 Commensalism nationallicence Cooperation nationallicence Flavonoids nationallicence Hydroxycinnamic acid nationallicence Parasitism nationallicence Rice secondary metabolite profiling nationallicence HCA : Hydroxycinnamic acid nationallicence PCA : Principal component analysis nationallicence PGPR : Plant growth-promoting rhizobacteria nationallicence Chamam Amel UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut Wisniewski-Dyé Florence UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut Comte Gilles UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut Bertrand Cédric Laboratoire de Chimie des Biomolécules et de l'Environnement EA 4215, Université de Perpignan, Via Domitia, 66860, Perpignan, France aut Prigent-Combaret Claire UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1439-1452 0032-0935 242:6<1439 2015 242 425 https://doi.org/10.1007/s00425-015-2382-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2382-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chamam Amel UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Wisniewski-Dyé Florence UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Comte Gilles UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Bertrand Cédric Laboratoire de Chimie des Biomolécules et de l'Environnement EA 4215, Université de Perpignan, Via Domitia, 66860, Perpignan, France aut NATIONALLICENCE 700 1- Prigent-Combaret Claire UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1439-1452 0032-0935 242:6<1439 2015 242 425