caa a22 4500 606207848 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2388-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2388-z Are trichomes involved in the biomechanical systems of Cucurbita leaf petioles? [Elektronische Daten] [Urszula Zajączkowska, Stanisław Kucharski, Dominika Guzek] Main conclusion : Trichomes are involved in petiole movement and likely function as a part of the plant biomechanical system serving as an additional reservoir of hydrostatic pressure. The large, non-glandular trichomes on Cucurbita petioles occur across collenchyma strands. Time-lapse imaging was used to study the leaf reorientation of Cucurbita maxima ‘Bambino' plants placed in horizontal position. The experiment comprised four variants of the large non-glandular petiole trichomes: (1) intact, (2) mechanically removed, (3) dehydrated, and (4) intact but with longitudinally injured petioles. Isolated strands of collenchyma with intact epidermis or epidermis mechanically removed from the abaxial and adaxial sides of the petiole were subjected to breaking test. The stiffness of the non-isolated tissue with intact epidermis was measured using the micro-indentation method. Petioles without trichomes did not exhibit tropic response, and the dehydration of trichomes slowed and prevented complete leaf reorientation. Isolated strands of collenchyma showed no correlation between strength values and position on the petiole. However, strands of collenchyma with epidermis exhibited a significantly greater strength regardless of their position on the petiole. The indentation test showed that non-isolated collenchyma is stiffer on the abaxial side of the petiole. Trichomes from the abaxial side of the petiole were larger at their base. The application of the ‘tensile triangles method' revealed that these trichomes had a biomechanically optimized shape in comparison to the adaxial side. We conclude that trichomes can be involved in plant biomechanical system and serve as an additional reservoir of hydrostatic pressure that is necessary for maintaining petioles in the prestressed state. The Author(s), 2015 Collenchyma nationallicence Epidermis nationallicence Plant biomechanics nationallicence Shape optimization nationallicence Tropic response nationallicence TS : Tissue stress nationallicence Zajączkowska Urszula Department of Forest Botany, Faculty of Forestry, Warsaw University of Life Sciences, 159 Nowoursynowska St., 02-776, Warsaw, Poland aut Kucharski Stanisław Department of Mechanics of Materials, Institute of Fundamental Technological Research, Polish Academy of Sciences, 5B Pawińskiego St., 02-106, Warsaw, Poland aut Guzek Dominika Division of Engineering in Nutrition, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02-776, Warsaw, Poland aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1453-1465 0032-0935 242:6<1453 2015 242 425 https://doi.org/10.1007/s00425-015-2388-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2388-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zajączkowska Urszula Department of Forest Botany, Faculty of Forestry, Warsaw University of Life Sciences, 159 Nowoursynowska St., 02-776, Warsaw, Poland aut NATIONALLICENCE 700 1- Kucharski Stanisław Department of Mechanics of Materials, Institute of Fundamental Technological Research, Polish Academy of Sciences, 5B Pawińskiego St., 02-106, Warsaw, Poland aut NATIONALLICENCE 700 1- Guzek Dominika Division of Engineering in Nutrition, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02-776, Warsaw, Poland aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1453-1465 0032-0935 242:6<1453 2015 242 425