caa a22 4500 606207856 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2373-6 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2373-6 Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots [Elektronische Daten] [Yanfeng Hu, Xiaofan Na, Jiaolong Li, Lijing Yang, Jia You, Xiaolei Liang, Jianfeng Wang, Liang Peng, Yurong Bi] Main conclusion : The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, has potential allelopathic activity and affects auxin transport. However, little is known about the cellular mechanism of this inhibitory effect of NCS on auxin transport. The present study characterizes the effects of NCS at the cellular level using transgenic Arabidopsis plants harboring the promoters of PIN, in combination with PIN-GFP proteins or AUX1-YFP fusions. NCS treatment caused significant reduction in the abundance of PIN and AUX1 proteins at the plasma membrane (PM). Analysis of the subcellular distribution of PIN and AUX1 proteins in roots revealed that NCS induced the intracellular accumulation of auxin transporters, including PIN2, PIN3, PIN4, PIN7 and AUX1. However, other PM proteins, such as PIP2, BRI1, and low temperature inducible protein 6b (LTI6b), were insensitive to NCS treatment. NCS-induced PIN2 compartments were further defined using endocytic tracer FM 4-64 labeled early endosomes and suggested that this compound affects the endocytosis trafficking of PIN proteins. Furthermore, pharmacological analysis indicated that the brefeldin A (BFA)-insensitive pathway is employed in the cellular effects of NCS on PIN2 trafficking. Although NCS did not alter actin dynamics in vitro, it resulted in the depolymerization of the actin cytoskeleton in vivo. This disruption of actin filaments by NCS subsequently influences the actin-based vesicle motility. Hence, the elucidation of the specific role of NCS is useful for further understanding the mechanisms of allelopathy at the phytohormone levels. Springer-Verlag Berlin Heidelberg, 2015 Allelochemical nationallicence Auxin nationallicence Auxin transport nationallicence Actin cytoskeleton nationallicence ARF : ADP-ribosylation factor family of GTP-binding proteins nationallicence AUX1/LAX : AUXIN RESISTANT1/LIKE AUXIN RESISTANT nationallicence BFA : Brefeldin A nationallicence eEF1A : Elongation factor one alpha nationallicence GEF : Guanine nucleotide exchange factor nationallicence LatB : Latrunculin B nationallicence NCS : Narciclasine nationallicence PIN : PIN-FORMED auxin efflux carrier protein nationallicence PM : Plasma membrane nationallicence TIBA : 2,3,5-Triiodobenzoic acid nationallicence TryA23 : Tyrphostin A23 nationallicence Hu Yanfeng Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Na Xiaofan Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Li Jiaolong Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Yang Lijing Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut You Jia School of Life Science, Northwest Normal University, 730070, Lanzhou, People's Republic of China aut Liang Xiaolei Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Wang Jianfeng Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Peng Liang Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Bi Yurong Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1349-1360 0032-0935 242:6<1349 2015 242 425 https://doi.org/10.1007/s00425-015-2373-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2373-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Yanfeng Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Na Xiaofan Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Jiaolong Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Lijing Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- You Jia School of Life Science, Northwest Normal University, 730070, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Xiaolei Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jianfeng Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Peng Liang Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Bi Yurong Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1349-1360 0032-0935 242:6<1349 2015 242 425