caa a22 4500 606207864 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00425-015-2366-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2366-5 Overexpression of a novel SbMYB15 from Salicornia brachiata confers salinity and dehydration tolerance by reduced oxidative damage and improved photosynthesis in transgenic tobacco [Elektronische Daten] [Pushp Shukla, Kapil Gupta, Parinita Agarwal, Bhavanath Jha, Pradeep Agarwal] Main conclusion : SbMYB15, R2R3-type MYB was induced by the different stresses, and conferred stress tolerance in transgenic tobacco by regulating the expression of stress-responsive genes. MYBs are the master regulators of various metabolic processes and stress responses in plants. In this study, we functionally characterised a R2R3-type SbMYB15 transcription factor (TF) from the extreme halophyte Salicornia brachiata. The SbMYB15 acts as a transcriptional activator. Transcriptional analysis showed that SbMYB15 transcript was strongly upregulated in red shoots and was also induced by different stresses; however, its expression remained unchanged with ABA. Overexpression of SbMYB15 in tobacco significantly improved salinity and dehydration tolerance. The enhanced tolerance of the transgenic plants was defined by the changes in chlorophyll, malondialdehyde (MDA), proline, total soluble sugar and total amino acid contents. The transgenic plants exhibited a higher membrane stability and reduced electrolyte leakage, H2O2 and O 2 − content compared to the wild type (WT). With ionic stress, transgenics showed a low Na+ and a high K+ content. In the transgenic plants, the expression of stress-responsive genes such as LEA5, ERD10D, PLC3, LTP1, HSF2, ADC, P5CS, SOD and CAT was enhanced in the presence of salinity, dehydration and heat. Exposure to gradual salinity and dehydration resulted in an increased stomatal conductance, water use efficiency, photosynthesis rate, photochemical quenching and reduced transpiration rate. Thus, SbMYB15 served as an important mediator of stress responses regulating different stress signalling pathways, leading to enhanced stress tolerance. Springer-Verlag Berlin Heidelberg, 2015 Abiotic stress tolerance nationallicence Halophyte nationallicence Osmolyte nationallicence Photosynthesis nationallicence R2R3-type MYB transcription factor nationallicence Transcription factor nationallicence ABA : Abscisic acid nationallicence Ci : Intercellular CO2 concentration nationallicence Ca : Ambient CO2 concentration nationallicence ETR : Photosynthetic electron transport rate nationallicence g : Stomatal conductance nationallicence MDA : Malondialdehyde nationallicence NPQ : Non-photochemical fluorescence quenching nationallicence PEG : Polyethylene glycol nationallicence SA : Salicylic acid nationallicence TF : Transcription factor nationallicence VA : Transgenic plants transformed with pCAMBIA 1301 nationallicence WUE : Water use efficiency nationallicence Shukla Pushp Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut Gupta Kapil Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut Agarwal Parinita Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut Jha Bhavanath Division of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364002, Bhavnagar, Gujarat, India aut Agarwal Pradeep Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1291-1308 0032-0935 242:6<1291 2015 242 425 https://doi.org/10.1007/s00425-015-2366-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2366-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shukla Pushp Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut NATIONALLICENCE 700 1- Gupta Kapil Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut NATIONALLICENCE 700 1- Agarwal Parinita Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut NATIONALLICENCE 700 1- Jha Bhavanath Division of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364002, Bhavnagar, Gujarat, India aut NATIONALLICENCE 700 1- Agarwal Pradeep Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, 364 002, Bhavnagar, Gujarat, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/6(2015-12-01), 1291-1308 0032-0935 242:6<1291 2015 242 425