caa a22 4500 606207902 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2367-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2367-4 Production of mono- and sesquiterpenes in Camelina sativa oilseed [Elektronische Daten] [Jörg Augustin, Yasuhiro Higashi, Xiaohong Feng, Toni Kutchan] Main conclusion : Camelina was bioengineered to accumulate (4 S )-limonene and (+)-δ-cadinene in seed. Plastidic localization of the recombinant enzymes resulted in higher yields than cytosolic localization. Overexpressing 1-deoxy- d -xylulose-5-phosphate synthase ( DXS ) further increased terpene accumulation. Many plant-derived compounds of high value for industrial or pharmaceutical applications originate from plant species that are not amenable to cultivation. Biotechnological production in low-input organisms is an attractive alternative. Several microbes are well established as biotechnological production platforms; however, their growth requires fermentation units, energy input, and nutrients. Plant-based production systems potentially allow the generation of high-value compounds on arable land with minimal input. Here we explore whether Camelina sativa (camelina), an emerging low-input non-foodstuff Brassicaceae oilseed crop grown on marginal lands or as a rotation crop on fallow land, can successfully be refactored to produce and store novel compounds in seed. As proof-of-concept, we use the cyclic monoterpene hydrocarbon (4S)-limonene and the bicyclic sesquiterpene hydrocarbon (+)-δ-cadinene, which have potential biofuel and industrial solvent applications. Post-translational translocation of the recombinant enzymes to the plastid with concurrent overexpression of 1-deoxy-d-xylulose-5-phosphate synthase (DXS) resulted in the accumulation of (4S)-limonene and (+)-δ-cadinene up to 7mgg−1 seed and 5mgg−1 seed, respectively. This study presents the framework for rapid engineering of camelina oilseed production platforms for terpene-based high-value compounds. Springer-Verlag Berlin Heidelberg, 2015 (+)-δ-Cadinene nationallicence Camelina nationallicence (4 S )-Limonene nationallicence Metabolic bioengineering nationallicence Terpenes nationallicence CDNS : Cadinene synthase nationallicence DMAPP : Dimethylallyl pyrophosphate nationallicence DXS : 1-Deoxy-d-xylulose-5-phosphate synthase nationallicence FAME : Fatty acid methyl ester nationallicence FDS : Farnesyl diphosphate synthase nationallicence FDS CDNS : Plant transformation construct containing FDS and CDNS ORFs nationallicence GDS : Geranyl diphosphate synthase nationallicence GDS LS : Plant transformation construct containing GDS and LS ORFs nationallicence GDSLS : Plant transformation construct containing GDSLS fusion ORF nationallicence GP : Glycinin promotor nationallicence GSL : Geranyl diphosphate synthase large subunit nationallicence GSS : Geranyl diphosphate synthase small subunit nationallicence GT : Glycinin terminator nationallicence IPP : Isopentenyl pyrophosphate nationallicence LS : Limonene synthase nationallicence MEP : 2-C-Methyl-d-erythritol 4-phosphate nationallicence NP : Napin promotor nationallicence OP : Oleosin promotor nationallicence ORF : Open reading frame nationallicence OT : Oleosin terminator nationallicence TP : Transit peptide nationallicence TPCDNS : Transit peptide cadinene synthase fusion nationallicence TPFDS : Transit peptide farnesyl diphosphate synthase fusion nationallicence TPFDS TPCDNS : Plant transformation construct containing TPFDS and TPCDNS ORFs nationallicence TPFDS TPCDNS DXS : Plant transformation construct containing TPFDS, TPCDNS, and DXS ORFs nationallicence TPGDS : Transit peptide geranyl diphosphate synthase fusion nationallicence TPGDS TPLS : Plant transformation construct containing TPGDS and TPLS ORFs nationallicence TPGDS TPLS DXS : Plant transformation construct containing TPGDS, TPLS, and DXS ORFs nationallicence TPGDSLS : Plant transformation construct containing TPGDSLS fusion ORF nationallicence TPLS : Transit peptide limonene synthase fusion nationallicence TPLSGDS : Plant transformation construct containing TPLSGDS fusion ORF nationallicence Augustin Jörg Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut Higashi Yasuhiro Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut Feng Xiaohong Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut Kutchan Toni Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 693-708 0032-0935 242:3<693 2015 242 425 https://doi.org/10.1007/s00425-015-2367-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2367-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Augustin Jörg Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Higashi Yasuhiro Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Feng Xiaohong Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Kutchan Toni Donald Danforth Plant Science Center, 975N Warson Road, 63132, St. Louis, MO, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 693-708 0032-0935 242:3<693 2015 242 425