caa a22 4500 606207929 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2370-9 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2370-9 Exploring the diversity in Citrus fruit colouration to decipher the relationship between plastid ultrastructure and carotenoid composition [Elektronische Daten] [Joanna Lado, Lorenzo Zacarías, Aranzazu Gurrea, Anton Page, Anthony Stead, María Rodrigo] Main conclusion : Differentiation of new and characteristic plastid ultrastructures during ripening of citrus fruits in both peel and pulp appears to be strongly correlated with the content and complement of carotenoids. Most of the species of the Citrus genus display a wide range in fruit colouration due to differences in carotenoids; however, how this diversity is related and may contribute to plastid differentiation and ultrastructure is currently unknown. To that end, carotenoid profile and plastid ultrastructure were compared in peel and pulp of three sweet oranges: the ordinary orange-coloured Navel, rich in β,β-xanthophylls, the yellow Pinalate mutant with an elevated content of colourless carotenes and reduced β,β-xanthophylls, and the red-fleshed Cara Cara with high concentration of colourless carotenes and lycopene in the pulp; and two grapefruits: the white Marsh, with low carotenoid content, and the red Star Ruby, accumulating upstream carotenes and lycopene. The most remarkable differences in plastid ultrastructure among varieties were detected in the pulp at full colour, coinciding with major differences in carotenoid composition. Accumulation of lycopene in Cara Cara and Star Ruby pulp was associated with the presence of needle-like crystals in the plastids, while high content of upstream carotenes in Pinalate pulp was related to the development of a novel plastid type with numerous even and round vesicles. The presence of plastoglobuli was linked to phytoene and xanthophyll accumulation, suggesting these structures as the main sites for the accumulation of these pigments. Peel chromoplasts were richer in membranes compared to pulp chromoplasts, reflecting their different biogenesis. In summary, differences in carotenoid composition and accumulation of unusual carotenoids are mirrored by the development of diverse and novel chromoplast types, revealing the plasticity of these organelles to rearrange carotenoids inside different structures to allow massive accumulation and thus contributing to the chemical stability of the carotenoids. Springer-Verlag Berlin Heidelberg, 2015 Carotenoids nationallicence Chloroplasts nationallicence Chromoplasts nationallicence Citrus fruit nationallicence Ripening nationallicence C, Cara : Cara orange nationallicence FW : Fresh weight nationallicence MSH : Marsh grapefruit nationallicence N : Navel orange nationallicence P : Pinalate orange nationallicence SR : Star Ruby grapefruit nationallicence TEM : Transmission electron microscopy nationallicence Lado Joanna Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Zacarías Lorenzo Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Gurrea Aranzazu Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Page Anton Biomedical Imaging Unit, Faculty of Medicine, Southampton General Hospital, University of Southampton, Tremona Road, Southampton, UK aut Stead Anthony School of Biological Sciences, Royal Holloway, University of London, Egham Hill, UK aut Rodrigo María Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 645-661 0032-0935 242:3<645 2015 242 425 https://doi.org/10.1007/s00425-015-2370-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2370-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lado Joanna Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 700 1- Zacarías Lorenzo Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 700 1- Gurrea Aranzazu Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 700 1- Page Anton Biomedical Imaging Unit, Faculty of Medicine, Southampton General Hospital, University of Southampton, Tremona Road, Southampton, UK aut NATIONALLICENCE 700 1- Stead Anthony School of Biological Sciences, Royal Holloway, University of London, Egham Hill, UK aut NATIONALLICENCE 700 1- Rodrigo María Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 645-661 0032-0935 242:3<645 2015 242 425