caa a22 4500 606207937 CHVBK 20210128101019.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2291-7 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2291-7 Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae) [Elektronische Daten] [Luc Bidel, Guillaume Chomicki, Fabien Bonini, Laurence Mondolot, Julien Soulé, Marc Coumans, Philippe La Fisca, Yves Baissac, Virginie Petit, Alain Loiseau, Zoran Cerovic, Kevin Gould, Christian Jay-Allemand] Main conclusion : A cumulative effect of UV-B doses on epidermal flavonol accumulation was observed during the first week of a time course study in Centella asiatica (Apiaceae). However, once flavonol levels had peaked, additional accumulation was possible only if higher daily UV-B irradiances were applied. We aimed to understand the dynamics of flavonol accumulation in leaf tissues using non-destructive spectroscopy and HPLC-mass spectrometry. When leaves that had grown without UV-B were given brief daily exposures to low-irradiance UV-B, they accumulated flavonols, predominantly kaempferol-3-O-β-d-glucuronopyranoside and quercetin-3-O-β-d-glucuronopyranoside, in their exposed epidermis, reaching a plateau after 7days. More prolonged UV-B exposures or higher doses eventually augmented flavonol concentrations even in non-exposed tissues. If UV-B irradiance was subsequently reduced, leaves appeared to lose their ability to accumulate further flavonols in their epidermis even if the duration of daily exposure was increased. A higher irradiance level was then necessary to further increase flavonol accumulation. When subsequently acclimated to higher UV-B irradiances, mature leaves accumulated less flavonols than did developing ones. Our study suggests that levels of epidermal flavonols in leaves are governed primarily by UV-B irradiance rather than by duration of exposure. European Union, 2015 Biospectroscopy nationallicence Centella asiatica nationallicence Dualex nationallicence Light acclimation nationallicence Polyphenols nationallicence UV-B absorption nationallicence Amu : Atomic mass unit nationallicence AU : Absorbance units nationallicence ChlF : Chlorophyll a fluorescence nationallicence ChlF(ex375), (ex655), : Chlorophyll a fluorescence induced by 375nm or by 655nm excitation wavelength nationallicence COP1 : CONSTITUTIVELY PHOTOMORPHOGENIC1 gene nationallicence DPBA : Diphenyl boric acid-2-aminoethyl ester nationallicence DA375 : Dualex absorbance at 375nm nationallicence LC-MS : Liquid chromatography-mass spectrometry nationallicence PAR : Photosynthetically active radiation nationallicence ROS : Reactive oxygen species nationallicence SIR : Single-ion record nationallicence RUP1 : REPRESSOR OF UV-B PHOTOMORPHOGENESIS1 protein nationallicence RUP2 : REPRESSOR OF UV-B PHOTOMORPHOGENESIS2 protein nationallicence UVR8 : UV RESISTANCE LOCUS8 gene nationallicence UV-A : Ultraviolet-A radiation (315-400nm) nationallicence UV-B : Ultraviolet-B radiation (280-315nm) nationallicence SAA : Systemic acquired acclimation nationallicence Bidel Luc INRA, UMR AGAP, Centre de Recherche de Montpellier, 2 Place Pierre Viala-Bât. 21, 34060, Montpellier, France aut Chomicki Guillaume Systematic Botany and Mycology, Department of Biology, University of Munich (LMU), 80638, Munich, Germany aut Bonini Fabien Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut Mondolot Laurence Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut Soulé Julien Centre d'Immunologie de Marseille-Luminy, Parc Scientifique & Technologique de Luminy, 13288, Marseille Cedex 09, France aut Coumans Marc UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut La Fisca Philippe Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut Baissac Yves UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut Petit Virginie Bayer HealthCare SAS Division Serdex, 1, Avenue Normandie-Niémen, 64140, Lons, France aut Loiseau Alain Bayer HealthCare SAS Division Serdex, 1, Avenue Normandie-Niémen, 64140, Lons, France aut Cerovic Zoran Equipe Biospectroscopie Végétale (CNRS), Laboratoire Ecologie Systématique Evolution, Département Ecophysiologie Végétale, UMR 8079 (CNRS, UPS, AgroParisTech), Université Paris-Sud 11, Orsay, France aut Gould Kevin School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand aut Jay-Allemand Christian UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 545-559 0032-0935 242:3<545 2015 242 425 https://doi.org/10.1007/s00425-015-2291-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2291-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bidel Luc INRA, UMR AGAP, Centre de Recherche de Montpellier, 2 Place Pierre Viala-Bât. 21, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Chomicki Guillaume Systematic Botany and Mycology, Department of Biology, University of Munich (LMU), 80638, Munich, Germany aut NATIONALLICENCE 700 1- Bonini Fabien Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Mondolot Laurence Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Soulé Julien Centre d'Immunologie de Marseille-Luminy, Parc Scientifique & Technologique de Luminy, 13288, Marseille Cedex 09, France aut NATIONALLICENCE 700 1- Coumans Marc UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut NATIONALLICENCE 700 1- La Fisca Philippe Laboratoire de Botanique, Phytochimie et Mycologie, UMR 5175 CEFE-CNRS, Faculté de Pharmacie, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Baissac Yves UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut NATIONALLICENCE 700 1- Petit Virginie Bayer HealthCare SAS Division Serdex, 1, Avenue Normandie-Niémen, 64140, Lons, France aut NATIONALLICENCE 700 1- Loiseau Alain Bayer HealthCare SAS Division Serdex, 1, Avenue Normandie-Niémen, 64140, Lons, France aut NATIONALLICENCE 700 1- Cerovic Zoran Equipe Biospectroscopie Végétale (CNRS), Laboratoire Ecologie Systématique Evolution, Département Ecophysiologie Végétale, UMR 8079 (CNRS, UPS, AgroParisTech), Université Paris-Sud 11, Orsay, France aut NATIONALLICENCE 700 1- Gould Kevin School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand aut NATIONALLICENCE 700 1- Jay-Allemand Christian UMR DIADE (UM2-IRD), SMART Team cc024, University of Montpellier, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 545-559 0032-0935 242:3<545 2015 242 425