caa a22 4500 606207988 CHVBK 20210128101020.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2308-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2308-2 Comparative analysis of antioxidant, antimicrobiological and cytotoxic activities of native and fermented chamomile ligulate flower extracts [Elektronische Daten] [Aleksandra Cvetanović, Jaroslava Švarc-Gajić, Zoran Zeković, Saša Savić, Jelena Vulić, Pavle Mašković, Gordana Ćetković] Main conclusion : The work investigated differences in apigenin content, as well as in other compounds, and examined the chemical profiles, antioxidant, antimicrobial and cytotoxic effects of extracts obtained from native and fermented chamomile ligulate flowers. Chamomile (Chamomilla recutita L.) has a long history of being used as a medicinal plant due to many health benefits, including antiinflammatory, anticancer, antispasmodic, radical-scavenging effects and others. Apigenin is recognized as one of the most bioactive phenolic compounds in chamomile. In comparison to its bound forms, which include mostly apigenin-7-O-β-glucoside and various acylated forms, the aglycone is attributed with much higher bioactivity. Due to this fact, in this work ligulate florets of chamomile anthodium were subjected to a fermentation process using native chamomile enzymes to hydrolyze bound forms of apigenin to free aglycone. The contents of apigenin and apigenin-7-O-β-glucoside were determined in both fermented and nonfermented samples by UHPLC-MS-MS analysis to define the efficiency of conversion. After defining their chemical profiles, the extracts of fermented and nonfermented chamomile samples were also compared with respect to their antioxidant, antimicrobial and cytotoxic effects. The antioxidant effects of the obtained extracts were defined by electron spin resonance analysis for hydroxyl and superoxide radicals. The antimicrobial activity was defined for eight microbial strains, whereas cytotoxic activity was evaluated using two human cell lines (human cervix carcinoma and human rhabdomyosarcoma) and murine fibroblasts. Springer-Verlag Berlin Heidelberg, 2015 Chamomile nationallicence Polyphenols nationallicence ESR spectroscopy nationallicence Cytotoxic activity nationallicence Antimicrobial activity nationallicence Cvetanović Aleksandra Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Švarc-Gajić Jaroslava Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Zeković Zoran Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Savić Saša Faculty of Technology, Bulevar Oslobođenja 124, 16000, Leskovac, Serbia aut Vulić Jelena Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Mašković Pavle Faculty of Agriculture, Cara Dušana 34, 32000, Čačak, Serbia aut Ćetković Gordana Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 721-732 0032-0935 242:3<721 2015 242 425 https://doi.org/10.1007/s00425-015-2308-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2308-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cvetanović Aleksandra Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 700 1- Švarc-Gajić Jaroslava Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 700 1- Zeković Zoran Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 700 1- Savić Saša Faculty of Technology, Bulevar Oslobođenja 124, 16000, Leskovac, Serbia aut NATIONALLICENCE 700 1- Vulić Jelena Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 700 1- Mašković Pavle Faculty of Agriculture, Cara Dušana 34, 32000, Čačak, Serbia aut NATIONALLICENCE 700 1- Ćetković Gordana Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 721-732 0032-0935 242:3<721 2015 242 425