caa a22 4500 606208003 CHVBK 20210128101020.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2352-y doi (NATIONALLICENCE)springer-10.1007/s00425-015-2352-y The extreme Albino3 (Alb3) C terminus is required for Alb3 stability and function in Arabidopsis thaliana [Elektronische Daten] [Manuela Urbischek, Sabine Nick von Braun, Thomas Brylok, Irene Gügel, Andreas Richter, Minna Koskela, Bernhard Grimm, Paula Mulo, Bettina Bölter, Jürgen Soll, Elisabeth Ankele, Serena Schwenkert] Main conclusion : The extreme Alb3 C terminus is important for Alb3 stability in a light dependent manner, but is dispensable for LHCP insertion or D1 synthesis. YidC/Oxa1/Alb3 dependent insertion of membrane proteins is evolutionary conserved among bacteria, mitochondria and chloroplasts. Chloroplasts are challenged by the need to coordinate membrane integration of nuclear encoded, post-translationally targeted proteins into the thylakoids as well as of proteins translated on plastid ribosomes. The pathway facilitating post-translational targeting of the light-harvesting chlorophyll a/b binding proteins involves the chloroplast signal recognition particle, cpSRP54 and cpSRP43, as well as its membrane receptor FtsY and the translocase Alb3. Interaction of cpSRP43 with Alb3 is mediated by the positively charged, stromal exposed C terminus of Alb3. In this study, we utilized an Alb3 T-DNA insertion mutant in Arabidopsis thaliana lacking the last 75 amino acids to elucidate the function of this domain (alb3∆C). However, the truncated Alb3 protein (Alb3∆C) proved to be unstable under standard growth conditions, resulting in a reduction of Alb3∆C to 20% of wild-type levels. In contrast, accumulation of Alb3∆C was comparable to wild type under low light growth conditions. Alb3∆C mutants grown under low light conditions were only slightly paler than wild type, accumulated almost wild-type levels of light harvesting proteins and were not affected in D1 synthesis, therefore showing that the extreme Alb3 C terminus is dispensable for both, co- and post-translational, protein insertion into the thylakoid membrane. However, reduction of Alb3∆C levels as observed under standard growth conditions resulted not only in a severely diminished accumulation of all thylakoid complexes but also in a strong defect in D1 synthesis and membrane insertion. Springer-Verlag Berlin Heidelberg, 2015 Chloroplast nationallicence Thylakoid membrane nationallicence Light harvesting complex nationallicence Photosystem II nationallicence Chlorophyll nationallicence Urbischek Manuela Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Nick von Braun Sabine Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Brylok Thomas Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Gügel Irene Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Richter Andreas Institute of Biology/Plant Physiology, Humboldt University, Philippstraße 13, 10115, Berlin, Germany aut Koskela Minna Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland aut Grimm Bernhard Institute of Biology/Plant Physiology, Humboldt University, Philippstraße 13, 10115, Berlin, Germany aut Mulo Paula Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland aut Bölter Bettina Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Soll Jürgen Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Ankele Elisabeth Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Schwenkert Serena Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 733-746 0032-0935 242:3<733 2015 242 425 https://doi.org/10.1007/s00425-015-2352-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2352-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Urbischek Manuela Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Nick von Braun Sabine Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Brylok Thomas Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Gügel Irene Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Richter Andreas Institute of Biology/Plant Physiology, Humboldt University, Philippstraße 13, 10115, Berlin, Germany aut NATIONALLICENCE 700 1- Koskela Minna Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland aut NATIONALLICENCE 700 1- Grimm Bernhard Institute of Biology/Plant Physiology, Humboldt University, Philippstraße 13, 10115, Berlin, Germany aut NATIONALLICENCE 700 1- Mulo Paula Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland aut NATIONALLICENCE 700 1- Bölter Bettina Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Soll Jürgen Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Ankele Elisabeth Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Schwenkert Serena Department Biologie I, Botanik, Ludwig-Maximilians-Universität, Großhaderner Strasse. 2-4, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 733-746 0032-0935 242:3<733 2015 242 425