caa a22 4500 606208046 CHVBK 20210128101020.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2343-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2343-z The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7 , is directly regulated by a rice transcription factor, OsMYB58/63 [Elektronische Daten] [Soichiro Noda, Taichi Koshiba, Takefumi Hattori, Masatoshi Yamaguchi, Shiro Suzuki, Toshiaki Umezawa] Main conclusion : A rice MYB transcription factor, OsMYB58/63, was found to directly upregulate the expression of a rice secondary wall-specific cellulose synthase gene, cellulose synthase A7 ( OsCesA7 ); in contrast, the Arabidopsis putative orthologs AtMYB58 and AtMYB63 have been shown to specifically activate lignin biosynthesis. Although indirect evidence has shown that grass plants are similar to but partially different from dicotyledonous ones in transcriptional regulation of lignocellulose biosynthesis, little is known about the differences. This study showed that a rice MYB transcription factor, OsMYB58/63, directly upregulated the expression of a rice secondary wall-specific cellulose synthase gene, cellulose synthase A7 (OsCesA7). Gene co-expression analysis showed that, in rice, OsMYB58/63 and several rice MYB genes were co-expressed with genes encoding lignocellulose biosynthetic enzymes. The expression levels of OsMYB55/61, OsMYB55/61-L, OsMYB58/63, and OsMYB42/85 were commonly found to be high in culm internodes and nodes. All four MYB transcription factors functioned as transcriptional activators in yeast cells. OsMYB58/63 most strongly transactivated the expression of OsCesA7 in rice protoplasts. Moreover, recombinant OsMYB58/63 protein was bound to two distinct cis-regulatory elements, AC-II and SMRE3, in the OsCesA7 promoter. This is in sharp contrast to the role of Arabidopsis orthologs, AtMYB58 and AtMYB63, which had been reported to specifically activate lignin biosynthesis. The promoter analysis revealed that AC elements, which are the binding sites for MYB58 and MYB63, were lacking in cellulose and xylan biosynthetic genes in Arabidopsis, but present in cellulose, xylan, and lignin biosynthetic genes in rice, implying that the difference of transcriptional regulation between rice and Arabidopsis is due to the distinct composition of promoters. Our results provide a new insight into transcriptional regulation in grass lignocellulose biosynthesis. Springer-Verlag Berlin Heidelberg, 2015 Cellulose nationallicence Lignin nationallicence Lignocellulose nationallicence Oryza sativa nationallicence Transcriptional regulation nationallicence Transcription factor nationallicence COR : Correlation coefficient nationallicence EMSA : Electrophoretic mobility shift assay nationallicence GAL4BD : GAL4 DNA-binding domain nationallicence OsCesA7 : Oryza sativa cellulose synthase A7 nationallicence OsCAldOMT1 : Oryza sativa 5-hydroxyconiferaldehyde O-methyltransferase 1 nationallicence OsGT43A : Oryza sativa glycosyltransferase family 43A nationallicence SCW : Secondary cell wall nationallicence TF : Transcription factor nationallicence Noda Soichiro Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Koshiba Taichi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Hattori Takefumi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Yamaguchi Masatoshi Institute for Environmental Science and Technology, Saitama University, 338-8570, Saitama, Japan aut Suzuki Shiro Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Umezawa Toshiaki Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 589-600 0032-0935 242:3<589 2015 242 425 https://doi.org/10.1007/s00425-015-2343-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2343-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Noda Soichiro Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Koshiba Taichi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Hattori Takefumi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masatoshi Institute for Environmental Science and Technology, Saitama University, 338-8570, Saitama, Japan aut NATIONALLICENCE 700 1- Suzuki Shiro Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Umezawa Toshiaki Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 589-600 0032-0935 242:3<589 2015 242 425