caa a22 4500 606208062 CHVBK 20210128101020.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00425-015-2325-1 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2325-1 Cloning and functional characterization of two monoterpene acetyltransferases from glandular trichomes of L. x intermedia [Elektronische Daten] [Lukman Sarker, Soheil Mahmoud] Main conclusion : Two alcohol acetyltransferases, LiAAT-3 and LiAAT-4, from L. x intermedia were cloned, expressed in bacteria, and functionally characterized. Two monoterpene acetyltransferase cDNA clones (LiAAT-3 and LiAAT-4) were isolated from L. x intermedia glandular trichomes, expressed in bacteria to produce, and functionally characterize the encoded proteins in vitro. The recombinant LiAAT-3 and LiAAT-4 proteins had molecular weights of ca. 47 and 49 kDa, respectively, as evidenced by SDS-PAGE. The K m (mM) values for the recombinant LiAAT-3 and LiAAT-4 were 1.046 and 0.354 for lavandulol, 1.31 and 0.279 for geraniol, and 0.87 and 0.113 for nerol, respectively. The V max (pkat/mg) values for LiAAT-3 and LiAAT-4 were 92.13 and 105.1 for lavandulol, 81.07 and 52.17 for geraniol, and 15.02 and 15.8 for nerol, correspondingly. Catalytic efficiencies (mM−1min−1) for LiAAT-3 and LiAAT-4 were 0.27 and 0.85 for lavandulol, 0.19 and 0.54 for geraniol, and 0.052 and 0.4 for nerol, respectively. These kinetic properties are in the range of those reported for other plant acetyltransferases, and indicate that LiAAT-4 has a better catalytic efficiency than LiAAT-3, with lavandulol serving as the preferred substrate for both enzymes. Transcripts for both genes were abundant in L. angustifolia and L. x intermedia flowers, where monoterpene acetates are produced, and were undetectable (or present in trace quantities) in L. latifolia flowers, which do not accumulate significant amounts of these metabolites. Springer-Verlag Berlin Heidelberg, 2015 Lavandula x intermedia nationallicence Essential oil nationallicence Alcohol acetyltransferase nationallicence Lavandulyl acetate nationallicence Geranyl acetate nationallicence Neryl acetate nationallicence Sarker Lukman Department of Biology, University of British Columbia, Okanagan Campus, 1177 Research Road, V1V 1V7, Kelowna, BC, Canada aut Mahmoud Soheil Department of Biology, University of British Columbia, Okanagan Campus, 1177 Research Road, V1V 1V7, Kelowna, BC, Canada aut Planta Springer Berlin Heidelberg 242/3(2015-09-01), 709-719 0032-0935 242:3<709 2015 242 425 https://doi.org/10.1007/s00425-015-2325-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2325-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarker Lukman Department of Biology, University of British Columbia, Okanagan Campus, 1177 Research Road, V1V 1V7, Kelowna, BC, Canada aut NATIONALLICENCE 700 1- Mahmoud Soheil Department of Biology, University of British Columbia, Okanagan Campus, 1177 Research Road, V1V 1V7, Kelowna, BC, Canada aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/3(2015-09-01), 709-719 0032-0935 242:3<709 2015 242 425