caa a22 4500 606208119 CHVBK 20210128101020.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2359-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2359-4 Insights into the multifaceted application of microscopic techniques in plant tissue culture systems [Elektronische Daten] [Mack Moyo, Adeyemi Aremu, Johannes Van Staden] Main conclusion : Microscopic techniques remain an integral tool which has allowed for the better understanding and manipulation of in vitro plant culture systems. The recent advancements will inevitably help to unlock the long-standing mysteries of fundamental biological mechanisms of plant cells. Beyond the classical applications in micropropagation aimed at the conservation of endangered and elite commercial genotypes, plant cell, tissue and organ cultures have become a platform for elucidating a myriad of fundamental physiological and developmental processes. In conjunction with microscopic techniques, in vitro culture technology has been at the centre of important breakthroughs in plant growth and development. Applications of microscopy and plant tissue culture have included elucidation of growth and development processes, detection of in vitro-induced physiological disorders as well as subcellular localization using fluorescent protein probes. Light and electron microscopy have been widely used in confirming the bipolarity of somatic embryos during somatic embryogenesis. The technique highlights basic anatomical, structural and histological evidence for in vitro-induced physiological disorders during plant growth and development. In this review, we discuss some significant biological insights in plant growth and development, breakthroughs and limitations of various microscopic applications and the exciting possibilities offered by emergent in vivo live imaging and fluorescent protein engineering technologies. Springer-Verlag Berlin Heidelberg, 2015 Fluorescent proteins nationallicence Histology nationallicence Organogenesis nationallicence Physiological disorders nationallicence Somatic embryogenesis nationallicence Subcellular localization nationallicence Moyo Mack Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Aremu Adeyemi Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Van Staden Johannes Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 773-790 0032-0935 242:4<773 2015 242 425 https://doi.org/10.1007/s00425-015-2359-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2359-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moyo Mack Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Aremu Adeyemi Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Van Staden Johannes Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 773-790 0032-0935 242:4<773 2015 242 425