caa a22 4500 60620816X CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2318-0 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2318-0 Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission [Elektronische Daten] [Tao Xu, Yanling Wang, Xin Liu, Shuangshuang Lv, Chaoyang Feng, Mingfang Qi, Tianlai Li] Main conclusion : We constructed small RNA and degradome sequencing libraries to identify miRNAs and targets involved in tomato pedicel abscission, and confirmed their roles via quantitative real-time PCR. MicroRNAs (miRNAs) are endogenous small RNAs which play crucial negatively regulatory roles at both the transcriptional and post-transcriptional levels in plants; however, limited knowledge is available on the expression profiles of miRNAs and their target genes during tomato pedicel abscission. Taking advantage of small RNA (sRNA) and degradome sequencing technology, a total of 56 known and 11 novel candidate miRNAs targeting 223 mRNA genes were confirmed during pedicel abscission. Gene ontology annotation and KEGG pathway analysis showed that these target genes were significantly enriched in intracellular, membrane-bounded organelle-related biological processes as well as in metabolic, plant-pathogen interaction and hormone signaling pathways. We screened 17 miRNA/target pairs for further analysis and performed quantitative real-time PCR to identify the roles. Cluster analysis of selected miRNAs revealed that the expression profiles of miRNAs varied in different stages of abscission and could be impacted by ethylene treatment. In the present study, the correlations between miRNAs and targets suggested a complex regulatory network of miRNA-mediated target interaction during pedicel abscission. Additionally, the expression profiles of miRNAs and their targets changed by ethylene might be a considerable reason why ethylene promotes pedicel abscission. Our study provides new insights into the expression and regulatory profiles of miRNAs during tomato pedicel abscission. Springer-Verlag Berlin Heidelberg, 2015 Ethylene nationallicence MicroRNA nationallicence Pedicel abscission nationallicence Tomato nationallicence ACS : 1-Aminocylopropane-1-carboxylic acid synthase nationallicence AGO1 : Argonaute1 nationallicence AP1G1 : AP-1 complex subunit gamma-1adaptins nationallicence AP2 : APETALA2 nationallicence AR : Abscission rate nationallicence ARF : Auxin response factor nationallicence AZ : Abscission zone nationallicence CDS : Coding sequence nationallicence CTR : Constitutive triple response nationallicence DCL1 : Dicer-like 1 nationallicence GO : Gene ontology nationallicence HD-ZIP : Homeodomain-leucine zipper nationallicence MC : MACROCALYX nationallicence MFE : Minimum free energy nationallicence OMTs : O-methyltransferases nationallicence PARE : Parallel analysis of RNA ends nationallicence PR : Pathogen related nationallicence SHAT1 : SHATTERINGABORTION1 nationallicence SPL : SQUAMOSA promoter-binding protein-like nationallicence sRNA : Small RNA nationallicence TAPG : Tomato abscission-related polygalacturonase nationallicence t-plot : Target plots nationallicence UTRs : Untranslated regions nationallicence Xu Tao College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Wang Yanling College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Liu Xin College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Lv Shuangshuang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Feng Chaoyang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Qi Mingfang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Li Tianlai College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 963-984 0032-0935 242:4<963 2015 242 425 https://doi.org/10.1007/s00425-015-2318-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2318-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Tao College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yanling College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xin College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Lv Shuangshuang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Feng Chaoyang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Qi Mingfang College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Li Tianlai College of Horticulture, Shenyang Agricultural University, 110866, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 963-984 0032-0935 242:4<963 2015 242 425