caa a22 4500 606208178 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2315-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2315-3 Physiological and biochemical mechanisms of the ornamental Eugenia myrtifolia L. plants for coping with NaCl stress and recovery [Elektronische Daten] [José-Ramón Acosta-Motos, Pedro Diaz-Vivancos, Sara Álvarez, Nieves Fernández-García, María Sanchez-Blanco, José Hernández] Main Conclusion : We studied the response of Eugenia myrtifolia L. plants, an ornamental shrub native to tropical and subtropical areas, to salt stress in order to facilitate the use of these plants in Mediterranean areas for landscaping. E. myrtifolia plants implement a series of adaptations to acclimate to salinity, including morphological, physiological and biochemical changes. Furthermore, the post-recovery period seems to be detected by Eugenia plants as a new stress situation. Different physiological and biochemical changes in Eugenia myrtifolia L. plants after being subjected to NaCl stress for up to 30days (Phase I) and after recovery from salinity (Phase II) were studied. Eugenia plants proved to be tolerant to NaCl concentrations between 44 and 88mM, displaying a series of adaptative mechanisms to cope with salt-stress, including the accumulation of toxic ions in roots. Plants increased their root/shoot ratio and decreased their leaf area, leaf water potential and stomatal conductance in order to limit water loss. In addition, they displayed different strategies to protect the photosynthetic machinery, including the limited accumulation of toxic ions in leaves, increase in chlorophyll content, changes in chlorophyll fluorescence parameters, leaf anatomy and antioxidant defence mechanisms. Anatomical modifications in leaves, including an increase in palisade parenchyma and intercellular spaces and decrease in spongy parenchyma, served to facilitate CO2 diffusion in a situation of reduced stomatal aperture. Salinity produced oxidative stress in Eugenia plants as evidenced by oxidative stress parameters values and a reduction in APX and ASC levels. Nevertheless, SOD and GSH contents increased. The post-recovery period is detected as a new stress situation, as observed through effects on plant growth and alterations in chlorophyll fluorescence and oxidative stress parameters. Springer-Verlag Berlin Heidelberg, 2015 ASC-GSH cycle nationallicence Gas exchange nationallicence Leaf anatomy nationallicence Oxidative stress nationallicence Recovery capacity nationallicence Water relations nationallicence APX : Ascorbate peroxidase nationallicence ASC : Ascorbate reduced form nationallicence DHAR : Dehydroascorbate reductase nationallicence GR : Glutathione reductase nationallicence GSH : Glutathione reduced form nationallicence GSSG : Glutathione oxidised form nationallicence MDHAR : Monodehydroascorbate reductase nationallicence POX : Peroxidase nationallicence SOD : Superoxide dismutase nationallicence Acosta-Motos José-Ramón Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Diaz-Vivancos Pedro Fruit Tree Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Álvarez Sara Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Fernández-García Nieves Department of Abiotic Stress and Plant Pathology, CEBAS-CSIC, P.O. Box 164, 30100, Murcia, Spain aut Sanchez-Blanco María Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Hernández José Fruit Tree Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 829-846 0032-0935 242:4<829 2015 242 425 https://doi.org/10.1007/s00425-015-2315-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2315-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Acosta-Motos José-Ramón Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Diaz-Vivancos Pedro Fruit Tree Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Álvarez Sara Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Fernández-García Nieves Department of Abiotic Stress and Plant Pathology, CEBAS-CSIC, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Sanchez-Blanco María Irrigation Department, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Hernández José Fruit Tree Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 829-846 0032-0935 242:4<829 2015 242 425