caa a22 4500 606208194 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2337-x doi (NATIONALLICENCE)springer-10.1007/s00425-015-2337-x The fungal endophyte Epichloë typhina improves photosynthesis efficiency of its host orchard grass ( Dactylis glomerata ) [Elektronische Daten] [Piotr Rozpądek, K. Wężowicz, M. Nosek, R. Ważny, K. Tokarz, M. Lembicz, Z. Miszalski, K. Turnau] Main conclusion : According to the results presented in this paper the fungal endophyte Epichloë typhina significantly improves the growth, PSII photochemistry and C assimilation efficiency of its host Dactylis glomerata. In this paper, we present a comprehensive study of the impact of the endophytic fungi Epichloë typhina on its plant hosts' photosynthesis apparatus. Chlorophyll a fluorescence, gas exchange, immuno-blotting and spectrophotometric measurements were employed to assess photosynthetic performance, changes in pigment content and mechanisms associated with light harvesting, carbon assimilation and energy distribution in Dactylis glomerata colonized with Epichloë typhina. According to the results presented in this study, colonization of D. glomerata results in improved photosynthesis efficiency. Additionally, we propose a new mechanism allowing plants to cope with the withdrawal of a significant fraction of its energy resources by the endophytic fungi. The abundance of LHCI, LHCII proteins as well as chlorophyll b was significantly higher in E+ plants. Malate export out of the chloroplast was shown to be increased in colonized plants. To our knowledge, we are the first to report this phenomenon. Epichloë colonization improved PSII photochemistry and C assimilation efficiency. Elevated energy demands of E+ D. glomerata plants are met by increasing the rate of carbon assimilation and PSII photochemistry. The Author(s), 2015 Endophyte nationallicence Dactylis glomerata nationallicence Epichloë typhina nationallicence Photosynthesis nationallicence NPQ nationallicence Rozpądek Piotr Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut Wężowicz K. Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut Nosek M. Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054, Kraków, Poland aut Ważny R. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Kraków, Poland aut Tokarz K. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland aut Lembicz M. Department of Plant Taxonomy, A. Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland aut Miszalski Z. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Kraków, Poland aut Turnau K. Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 1025-1035 0032-0935 242:4<1025 2015 242 425 https://doi.org/10.1007/s00425-015-2337-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2337-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rozpądek Piotr Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut NATIONALLICENCE 700 1- Wężowicz K. Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut NATIONALLICENCE 700 1- Nosek M. Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054, Kraków, Poland aut NATIONALLICENCE 700 1- Ważny R. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Kraków, Poland aut NATIONALLICENCE 700 1- Tokarz K. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland aut NATIONALLICENCE 700 1- Lembicz M. Department of Plant Taxonomy, A. Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland aut NATIONALLICENCE 700 1- Miszalski Z. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Kraków, Poland aut NATIONALLICENCE 700 1- Turnau K. Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 1025-1035 0032-0935 242:4<1025 2015 242 425