caa a22 4500 606208216 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2324-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2324-2 Mutations and amplification of EPSPS gene confer resistance to glyphosate in goosegrass ( Eleusine indica ) [Elektronische Daten] [Jingchao Chen, Hongjuan Huang, Chaoxian Zhang, Shouhui Wei, Zhaofeng Huang, Jinyi Chen, Xu Wang] Main conclusion : Field-evolved resistance of goosegrass to glyphosate is due to double or single mutation in EPSPS , or amplification of EPSPS leads to increased transcription and protein levels. Glyphosate has been used widely in the south of China. The high selection pressure from glyphosate use has led to the evolution of resistance to glyphosate in weeds. We investigated the molecular mechanisms of three recently discovered glyphosate-resistant Eleusine indica populations (R1, R2 and R3). The results showed that R1 and R2 had double Thr102Ile and Pro106Ser mutation and a single mutation of Pro106Leu in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, respectively. Escherichia coli containing the mutated EPSPS genes was tolerant to glyphosate. EPSPS activity in R1 and R2 plants was higher than in the sensitive plants. There was no amino acid substitution in EPSPS gene in R3. However, expression of EPSPS in R3 plants was higher than in glyphosate-susceptible (S) population (13.8-fold) after glyphosate treatment. EPSPS enzyme activity in both R3 and S plants was inhibited by glyphosate, while shikimate accumulation in R3 was significantly lower than for the S population. Further analysis revealed that the genome of R3 contained 28.3-fold more copies of the EPSPS gene than that of susceptible population. EPSPS expression was positively correlated with copy number of EPSPS. In conclusion, mutation of the EPSPS gene and increased EPSPS expression are part of the molecular mechanisms of resistance to glyphosate in Eleusine indica. Springer-Verlag Berlin Heidelberg, 2015 Glyphosate nationallicence Resistance nationallicence EPSPS nationallicence Eleusine indica nationallicence Mechanism nationallicence EPSPS : 5-enolpyruvylshikimate-3-phosphate synthase nationallicence GR : Glyphosate-resistant nationallicence S : Glyphosate-susceptible nationallicence PCR : Polymerase chain reaction nationallicence Chen Jingchao Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Huang Hongjuan Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Zhang Chaoxian Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Wei Shouhui Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Huang Zhaofeng Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Chen Jinyi Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Wang Xu Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 859-868 0032-0935 242:4<859 2015 242 425 https://doi.org/10.1007/s00425-015-2324-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2324-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jingchao Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Huang Hongjuan Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhang Chaoxian Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Wei Shouhui Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Huang Zhaofeng Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Chen Jinyi Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 700 1- Wang Xu Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 859-868 0032-0935 242:4<859 2015 242 425