caa a22 4500 606208240 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2322-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2322-4 Misexpression of the Niemann-Pick disease type C1 (NPC1)-like protein in Arabidopsis causes sphingolipid accumulation and reproductive defects [Elektronische Daten] [Maximilian Feldman, Brenton Poirier, B. Lange] Main conclusion : Misexpression of the AtNPC1 - 1 and AtNPC1 - 2 genes leads to altered sphingolipid metabolism, growth impairment, and male reproductive defects in a hemizygous Arabidopsis thaliana (L.) double-mutant population. Abolishing the expression of both gene copies has lethal effects. Abstract: Niemann-Pick disease type C1 is a lysosomal storage disorder caused by mutations in the NPC1 gene. At the cellular level, the disorder is characterized by the accumulation of storage lipids and lipid trafficking defects. The Arabidopsis thaliana genome contains two genes (At1g42470 and At4g38350) with weak homology to mammalian NPC1. The corresponding proteins have 11 predicted membrane-spanning regions and contain a putative sterol-sensing domain. The At1g42470 protein is localized to the plasma membrane, while At4g38350 protein has a dual localization in the plasma and tonoplast membranes. A phenotypic analysis of T-DNA insertion mutants indicated that At1g42470 and At4g38350 (designated AtNPC1-1 and AtNPC1-2, respectively) have partially redundant functions and are essential for plant reproductive viability and development. Homozygous plants impaired in the expression of both genes were not recoverable. Plants of a hemizygous AtNPC1-1/atnpc1-1/atnpc1-2/atnpc1-2 population were severely dwarfed and exhibited male gametophytic defects. These gene disruptions did not have an effect on sterol concentrations; however, hemizygous AtNPC1-1/atnpc1-1/atnpc1-2/atnpc1-2 mutants had increased fatty acid amounts. Among these, fatty acid α-hydroxytetracosanoic acid (h24:0) occurs in plant sphingolipids. Follow-up analyses confirmed the accumulation of significantly increased levels of sphingolipids (assayed as hydrolyzed sphingoid base component) in the hemizygous double-mutant population. Certain effects of NPC1 misexpression may be common across divergent lineages of eukaryotes (sphingolipid accumulation), while other defects (sterol accumulation) may occur only in certain groups of eukaryotic organisms. Springer-Verlag Berlin Heidelberg, 2015 Male gametophyte nationallicence Niemann-Pick disease type C1 nationallicence Sphingolipid nationallicence Sterol-sensing domain nationallicence GC-MS : Gas chromatography-mass spectrometry nationallicence GO : Gene ontology nationallicence HMGR : 3-hydroxy-3-methylglutaryl-coenzyme A reductase nationallicence LE/Lys : Late endosomal/lysosomal compartment nationallicence NPC1 : Niemann-Pick disease type C1 nationallicence SBH : Sphingoid base hydroxylase nationallicence SCAP : SREBP cleavage activating protein nationallicence SPT : Serine palmitoyltransferase nationallicence SREBP : Sterol regulatory element binding protein nationallicence SSD : Sterol-sensing domain nationallicence Feldman Maximilian Donald Danforth Plant Science Center, 975 North Warson Road, 63132, St. Louis, MO, USA aut Poirier Brenton Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, PO Box 646340, 99164-6340, Pullman, WA, USA aut Lange B. Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, PO Box 646340, 99164-6340, Pullman, WA, USA aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 921-933 0032-0935 242:4<921 2015 242 425 https://doi.org/10.1007/s00425-015-2322-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2322-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feldman Maximilian Donald Danforth Plant Science Center, 975 North Warson Road, 63132, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Poirier Brenton Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, PO Box 646340, 99164-6340, Pullman, WA, USA aut NATIONALLICENCE 700 1- Lange B. Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, PO Box 646340, 99164-6340, Pullman, WA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 921-933 0032-0935 242:4<921 2015 242 425