caa a22 4500 606208259 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2328-y doi (NATIONALLICENCE)springer-10.1007/s00425-015-2328-y Brassinosteroid is required for sugar promotion of hypocotyl elongation in Arabidopsis in darkness [Elektronische Daten] [Yongqiang Zhang, Zhongjuan Liu, Jianfeng Wang, Yadi Chen, Yurong Bi, Junxian He] Main Conclusion : Brassinosteroid is necessary for sugar promotion of Arabidopsis hypocotyl elongation in darkness, and sugar positively regulates BRASSINAZOLE RESISTANT1 (BZR1) at both transcription and protein levels. Sugar has the ability to induce Arabidopsis hypocotyl elongation in the dark, but the detailed mechanisms remain not well understood. Here, we report that the steroidal phytohormone brassinosteroid (BR) is involved in sugar promotion of hypocotyl elongation in the dark. Sugar-induced hypocotyl elongation was significantly repressed in the BR-deficient mutant det2-1, BR-insensitive mutant bri1-5, and wild-type plants (Col-0), but not in the BR-hypersensitive mutants bzr1-1D and bes1-D treated with the BR biosynthetic inhibitor brassinazole (BRZ). Sugar also up-regulated the expression of genes that are related to cell elongation in a BR-dependent manner, and this effect was more remarkable in bzr1-1D and bes1-D than in their corresponding wild types in the presence of BRZ, suggesting an important role of BZR1 and bri1-ems-suppressor 1 (BES1) in this process. Sugar treatment seems to have little effect on BR biosynthesis, but enhances the expression of BZR1 and BES1, two transcription factors in BR signaling, in the dark. Furthermore, sugar treatment maintains higher BZR1 protein levels in plants grown in the dark. Collectively, our results indicate that BR is required for sugar promotion of hypocotyl elongation in darkness in Arabidopsis. Springer-Verlag Berlin Heidelberg, 2015 Brassinolide nationallicence Brassinosteroid nationallicence Dark nationallicence Hypocotyl elongation nationallicence Signaling nationallicence Sugar nationallicence BES1 : Bri1-ems-suppressor 1 nationallicence BL : Brassinolide nationallicence BR : Brassinosteroid nationallicence bri1 : Brassinosteroid insensitive 1 nationallicence BRZ : Brassinazole nationallicence BZR1 : Brassinazole resistant 1 nationallicence CPD : Constitutive photomorphogenic dwarf nationallicence det2 : De-etiolated 2 nationallicence DWF4 : Dwarf 4 nationallicence EXPA8 : Expansin A8 nationallicence EXPL2 : Expansin-like A2 nationallicence Glc : Glucose nationallicence IAA19 : Indole-3-acetic inducible 19 nationallicence Mtl : Mannitol nationallicence PP2A : Protein phosphatase 2A nationallicence PRE1 : Paclobutrazol resistance 1 nationallicence PRE5 : Paclobutrazol resistance 5 nationallicence qRT-PCR : Quantitative reverse transcription polymerase chain reaction nationallicence Suc : Sucrose nationallicence XTH18 : Xyloglucan endotransglucosylase/hydrolase 18 nationallicence Zhang Yongqiang School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Liu Zhongjuan State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut Wang Jianfeng Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut Chen Yadi Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut Bi Yurong Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut He Junxian School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 881-893 0032-0935 242:4<881 2015 242 425 https://doi.org/10.1007/s00425-015-2328-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2328-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yongqiang School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Liu Zhongjuan State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jianfeng Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yadi Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut NATIONALLICENCE 700 1- Bi Yurong Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, Gansu, People's Republic of China aut NATIONALLICENCE 700 1- He Junxian School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 881-893 0032-0935 242:4<881 2015 242 425