caa a22 4500 606208267 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2335-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2335-z DArT-based characterisation of genetic diversity in a Miscanthus collection from Poland [Elektronische Daten] [Jie Tang, Maurycy Daroch, Andrzej Kilian, Stanislaw Jeżowski, Marta Pogrzeba, Michal Mos] Main conclusion : Analysis of 180 accessions of Miscanthus using a DArT platform revealed high diversity. The phylogenetic analysis revealed that M.×giganteus accessions fall into two genetically distinct groups. Miscanthus is a genus of perennial rhizomatous grasses that has emerged in last 20years as a feedstock for bioenergy and biofuel production. Currently, the most widely used accession for bioenergy purposes is Miscanthus×giganteus, a sterile triploid hybrid between Miscanthus sinensis and Miscanthus sacchariflorus. However, previous reports have shown that genetic diversity of Miscanthus×giganteus is limited. Here, we report development of Diversity Arrays Technology platform for the analysis of genetic structure of a Miscanthus collection of 180 accessions. A total of 906 markers were obtained of which around 25.5% exhibited polymorphism information content value in the range of 0.40 and 0.50 and are considered particularly informative. Newly developed marker system will serve as an additional resource to assist crop improvement, germplasm preservation and genetic studies. Three types of analysis indicated that 180 accessions from the collection were well differentiated and presented high diversity. Interestingly, the analysis revealed that there are two separate groups of plants, significantly differing in genetic diversity, that are commercially available as M.×giganteus. We suggest that one of these groups is most likely mutants or somaclonal variants of original M.×giganteus. The other group is recent hybrids of Miscanthus of higher genetic diversity. This study indicates that the diversity of commercially available M.×giganteus is higher than commonly assumed. Development of the new marker system can significantly assist breeding of new commercial cultivars of Miscanthus for bioenergy use. Springer-Verlag Berlin Heidelberg, 2015 DArT nationallicence Genetic diversity nationallicence Microarray nationallicence Miscanthus nationallicence Population structure nationallicence AMOVA : Analysis of molecular variance nationallicence DArT : Diversity arrays technology nationallicence PCoA : Principal coordinate analysis nationallicence PIC : Polymorphism information content nationallicence RAD-seq : Restriction site associated DNA sequencing nationallicence UPGMA : Unweighted pair group method with algorithmic mean nationallicence Tang Jie Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut Daroch Maurycy Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut Kilian Andrzej Diversity Array Technology Pty Ltd, Bldg 3 Lvl D, University of Canberra, Kirinari St, 2617, Bruce, ACT, Australia aut Jeżowski Stanislaw Institute of Plant Genetics, Polish Academy of Science, Strzeszynska 34, 60-479, Poznan, Poland aut Pogrzeba Marta Departments of Environmental Biotechnology, Phytoremediation Team, Institute for Ecology of Industrial Areas (IETU), Kossutha 6, 40-844, Katowice, Poland aut Mos Michal Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 985-996 0032-0935 242:4<985 2015 242 425 https://doi.org/10.1007/s00425-015-2335-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2335-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang Jie Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut NATIONALLICENCE 700 1- Daroch Maurycy Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut NATIONALLICENCE 700 1- Kilian Andrzej Diversity Array Technology Pty Ltd, Bldg 3 Lvl D, University of Canberra, Kirinari St, 2617, Bruce, ACT, Australia aut NATIONALLICENCE 700 1- Jeżowski Stanislaw Institute of Plant Genetics, Polish Academy of Science, Strzeszynska 34, 60-479, Poznan, Poland aut NATIONALLICENCE 700 1- Pogrzeba Marta Departments of Environmental Biotechnology, Phytoremediation Team, Institute for Ecology of Industrial Areas (IETU), Kossutha 6, 40-844, Katowice, Poland aut NATIONALLICENCE 700 1- Mos Michal Energene sp. z o.o., Wróblewskiego 38A, 93-578, Lodz, Poland aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 985-996 0032-0935 242:4<985 2015 242 425