caa a22 4500 606208275 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2317-1 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2317-1 Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley [Elektronische Daten] [Honghong Wu, Lana Shabala, Meixue Zhou, Giovanni Stefano, Camilla Pandolfi, Stefano Mancuso, Sergey Shabala] Main conclusion : Leaf tissue tolerance was strongly and positively correlated with overall salt tolerance in barley, but not in wheat where the inability of sensitive varieties to exclude Na + is compensated by their better ability to handle Na + accumulated in the shoot via tissue tolerance mechanisms. A new high-throughput assay was developed to use the excised leaves to eliminate the confounding contribution of sodium exclusion mechanisms and evaluate genetic variability in salinity tissue tolerance in a large number of wheat (Triticum aestivum and Triticum turgidum ssp. durum) and barley (Hordeum vulgare) accessions. The changes in relative chlorophyll content (measured as chlorophyll content index, CCI) in excised leaves exposed to 50mM NaCl for 48h were found to be a reliable indicator of leaf tissue tolerance. In both wheat and barley, relative CCI correlated strongly with the overall plant salinity tolerance (evaluated in glasshouse experiments). To a large extent, this tissue tolerance was related to more efficient vacuolar Na+ sequestration in leaf mesophyll, as revealed by fluorescent Na+ dye imaging experiments. However, while in barley this correlation was positive, tissue tolerance in wheat correlated negatively with overall salinity tolerance. As a result, more salt-sensitive durum wheat genotypes possessed higher tissue tolerance than bread wheat plants, and this negative correlation was present within each of bread and durum wheat clusters as well. Overall, these results indicate that the lack of effective Na+ exclusion ability in sensitive wheat varieties is compensated by their better ability to handle Na+ accumulated in the shoot via tissue tolerance mechanisms. Implications of these findings for plant breeding for salinity tolerance are discussed. Springer-Verlag Berlin Heidelberg, 2015 Barley nationallicence Chlorophyll content nationallicence Excised leaf nationallicence Tissue tolerance nationallicence Vacuolar Na+ sequestration nationallicence Wheat nationallicence Triticum aestivum nationallicence Triticum turgidum ssp. durum nationallicence Hordeum vulgare nationallicence CCI : Chlorophyll content index nationallicence FV : Na+ permeable fast-activating tonoplast channels nationallicence HKT : High affinity K+ transporter nationallicence MAS : Marker assisted selection nationallicence NHX : Na+/H+ exchanger nationallicence ROS : Reactive oxygen species nationallicence SOS : Salt overly sensitive nationallicence SV : Slow-activating vacuolar channels nationallicence Wu Honghong School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Shabala Lana School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Zhou Meixue School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Stefano Giovanni MSU DOE Plant Research Laboratory, Michigan State University, 48824, East Lansing, MI, USA aut Pandolfi Camilla Department of Horticulture, University of Florence, 50019, Sesto Fiorentino, Italy aut Mancuso Stefano Department of Horticulture, University of Florence, 50019, Sesto Fiorentino, Italy aut Shabala Sergey School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 847-857 0032-0935 242:4<847 2015 242 425 https://doi.org/10.1007/s00425-015-2317-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2317-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Honghong School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Shabala Lana School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Zhou Meixue School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Stefano Giovanni MSU DOE Plant Research Laboratory, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Pandolfi Camilla Department of Horticulture, University of Florence, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Mancuso Stefano Department of Horticulture, University of Florence, 50019, Sesto Fiorentino, Italy aut NATIONALLICENCE 700 1- Shabala Sergey School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 847-857 0032-0935 242:4<847 2015 242 425