caa a22 4500 606208283 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00425-015-2316-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2316-2 Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach [Elektronische Daten] [Branka Živanović, Lana Shabala, Theo Elzenga, Sergey Shabala] Main conclusion : Blue light signalling pathway in broad bean leaf epidermal cells includes key membrane transporters: plasma- and endomembrane channels and pumps of H + , Ca 2+ and K + ions, and plasma membrane redox system. Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on-off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction. Springer-Verlag Berlin Heidelberg, 2015 Broad bean nationallicence Inhibitors nationallicence Ion fluxes nationallicence Microelectrodes nationallicence BL : Blue light nationallicence CaM : Calmodulin nationallicence CRY : Cryptochrome nationallicence DPI : Diphenyleneiodonium nationallicence ErB : Erythrocin B nationallicence EY : Eosin yellow nationallicence HCFIII : Hexacyanoferrate (III) nationallicence MIFE : Microelectrode ion flux estimation nationallicence NM : Neomicyn nationallicence PHOT : Phototropin nationallicence RR : Ruthenium red nationallicence TG : Thapsigargin nationallicence TFP : Trifluoperazine nationallicence TMB-8 : 8-(N,N-diethylamino) octyl-3,4,5-trimethoxybenzoate nationallicence W7 : N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide nationallicence Živanović Branka School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Shabala Lana School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Elzenga Theo Ecophysiology of Plants, University of Groningen, Nijenborgh 7, Groningen, The Netherlands aut Shabala Sergey School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut Planta Springer Berlin Heidelberg 242/4(2015-10-01), 813-827 0032-0935 242:4<813 2015 242 425 https://doi.org/10.1007/s00425-015-2316-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2316-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Živanović Branka School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Shabala Lana School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Elzenga Theo Ecophysiology of Plants, University of Groningen, Nijenborgh 7, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Shabala Sergey School of Land and Food, University of Tasmania, Private Bag 54, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/4(2015-10-01), 813-827 0032-0935 242:4<813 2015 242 425