caa a22 4500 606208305 CHVBK 20210128101021.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2353-x doi (NATIONALLICENCE)springer-10.1007/s00425-015-2353-x DNA barcodes for the identification of Anoectochilus roxburghii and its adulterants [Elektronische Daten] [Tongwei Lv, Renda Teng, Qingsong Shao, Hongzhen Wang, Wangshu Zhang, Mingyan Li, Lili Zhang] Main conclusion : Chinese medicinal herbs have a similar appearance and are easily confused, complicating identification via traditional methods. This study provided a scientific approach, based on DNA barcoding, to accurately and rapidly identify Anoectochilus roxburghii and its adulterants. This technology complements traditional methods of identification of medicinal herbs. A comparison of the DNA barcodes matK, psbA-trnH and ITS2 was performed to verify that the ITS2 sequence is an effective marker for rapidly and accurately identifying A. roxburghii and its closely related species. Genomic DNA extracted from A. roxburghii and its adulterants were used as templates and the ITS2 sequence was amplified using PCR amplification and sequencing. Species identification was conducted using BLAST1 and neighbor-joining trees. The 12 samples were successfully classified into four species based on the ITS2 sequence. The ITS2 sequence length of A. roxburghii was 253bp. The average intra-specific genetic distance of A. roxburghii was 0.0021, markedly lower than the inter-specific genetic distance between A. roxburghii and its adulterants (0.0380). Our findings illustrate that ITS2 sequence can accurately and efficiently distinguish A. roxburghii and its adulterants. In addition, the results provided reference for molecular identification of other Chinese herbal medicine. Springer-Verlag Berlin Heidelberg, 2015 Anoectochilus roxburghii nationallicence ITS2 nationallicence Sequence analysis nationallicence Molecular identification nationallicence ITS2 : Internal transcribed spacer 2 nationallicence PCR : Polymerase chain reaction nationallicence TBE : Tris-borate-EDTA nationallicence NJ : Tree neighbor-joining tree nationallicence TLC : Thin layer chromatography nationallicence HMM : Hidden Markov model nationallicence Lv Tongwei The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut Teng Renda The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut Shao Qingsong The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut Wang Hongzhen The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut Zhang Wangshu Modern Agricultural Research Center in Ningbo, Industrial Technology Research Institute of Zhejiang University, 315033, Ningbo, China aut Li Mingyan Zhejiang Rare Herbal Medicine and Engineering Institute, 321200, Wuyi, China aut Zhang Lili The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1167-1174 0032-0935 242:5<1167 2015 242 425 https://doi.org/10.1007/s00425-015-2353-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2353-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lv Tongwei The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut NATIONALLICENCE 700 1- Teng Renda The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut NATIONALLICENCE 700 1- Shao Qingsong The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut NATIONALLICENCE 700 1- Wang Hongzhen The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Wangshu Modern Agricultural Research Center in Ningbo, Industrial Technology Research Institute of Zhejiang University, 315033, Ningbo, China aut NATIONALLICENCE 700 1- Li Mingyan Zhejiang Rare Herbal Medicine and Engineering Institute, 321200, Wuyi, China aut NATIONALLICENCE 700 1- Zhang Lili The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 311300, Hangzhou, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1167-1174 0032-0935 242:5<1167 2015 242 425