caa a22 4500 606208348 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2339-8 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2339-8 BTH and BABA induce resistance in pea against rust ( Uromyces pisi ) involving differential phytoalexin accumulation [Elektronische Daten] [Eleonora Barilli, Diego Rubiales, Carmine Amalfitano, Antonio Evidente, Elena Prats] Main Conclusion : Systemic acquired resistance elicitors, BTH and BABA, reduce rust penetration in pea through phytoalexins pathway but differing in their mode of action. It has been previously shown that rust (Uromyces pisi) infection can be reduced in pea (Pisum sativum) by exogenous applications of systemic acquired resistance elicitors such as BTH and BABA. This protection is known to be related with the induction of the phenolic pathway but the particular metabolites involved have not been determined yet. In this work, we tackled the changes induced in phytoalexin content by BTH and BABA treatments in the context of the resistance responses to pea rust. Detailed analysis through high-performance liquid chromatography (HPLC) showed qualitative and quantitative differences in the content, as well as in the distribution of phytoalexins. Thus, following BTH treatment, we observed an increase in scopoletin, pisatin and medicarpin contents in all, excreted, soluble and cell wall-bound fraction. This suggests fungal growth impairment by both direct toxic effect as well as plant cell wall reinforcement. The response mediated by BTH was genotype-dependent, since coumarin accumulation was observed only in the resistant genotype whereas treatment by BABA primed phytoalexin accumulation in both genotypes equally. Exogenous application to the leaves of scopoletin, medicarpin and pisatin lead to a reduction of the different fungal growth stages, confirming a role for these phytoalexins in BTH- and BABA-induced resistance against U. pisi hampering pre- and postpenetration fungal stages. Springer-Verlag Berlin Heidelberg, 2015 Elicitors nationallicence Fungal impairment nationallicence Pea rust nationallicence Phytoalexins nationallicence BABA : (D,L)-3-amino-n-butanoic ((D,L)-β-aminobutyric) acid nationallicence BTH : Benzo[1,2,3]thiadiazole-7-carbothionic acid-S-methyl ester (Bion®) nationallicence SAR : Systemic acquired resistance nationallicence Barilli Eleonora Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut Rubiales Diego Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut Amalfitano Carmine Agrarian Department, University of Naples Federico II, Portici, Italy aut Evidente Antonio Department of Chemical Science, University of Naples Federico II, Naples, Italy aut Prats Elena Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1095-1106 0032-0935 242:5<1095 2015 242 425 https://doi.org/10.1007/s00425-015-2339-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2339-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barilli Eleonora Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut NATIONALLICENCE 700 1- Rubiales Diego Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut NATIONALLICENCE 700 1- Amalfitano Carmine Agrarian Department, University of Naples Federico II, Portici, Italy aut NATIONALLICENCE 700 1- Evidente Antonio Department of Chemical Science, University of Naples Federico II, Naples, Italy aut NATIONALLICENCE 700 1- Prats Elena Institute for Sustainable Agriculture, CSIC, Córdoba, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1095-1106 0032-0935 242:5<1095 2015 242 425