caa a22 4500 606208356 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2344-y doi (NATIONALLICENCE)springer-10.1007/s00425-015-2344-y Xyloglucan, galactomannan, glucuronoxylan, and rhamnogalacturonan I do not have identical structures in soybean root and root hair cell walls [Elektronische Daten] [Artur Muszyński, Malcolm O'Neill, Easwaran Ramasamy, Sivakumar Pattathil, Utku Avci, Maria Peña, Marc Libault, Md Hossain, Laurent Brechenmacher, William York, Rommel Barbosa, Michael Hahn, Gary Stacey, Russell Carlson] Main conclusion : Chemical analyses and glycome profiling demonstrate differences in the structures of the xyloglucan, galactomannan, glucuronoxylan, and rhamnogalacturonan I isolated from soybean ( Glycine max ) roots and root hair cell walls. The root hair is a plant cell that extends only at its tip. All other root cells have the ability to grow in different directions (diffuse growth). Although both growth modes require controlled expansion of the cell wall, the types and structures of polysaccharides in the walls of diffuse and tip-growing cells from the same plant have not been determined. Soybean (Glycine max) is one of the few plants whose root hairs can be isolated in amounts sufficient for cell wall chemical characterization. Here, we describe the structural features of rhamnogalacturonan I, rhamnogalacturonan II, xyloglucan, glucomannan, and 4-O-methyl glucuronoxylan present in the cell walls of soybean root hairs and roots stripped of root hairs. Irrespective of cell type, rhamnogalacturonan II exists as a dimer that is cross-linked by a borate ester. Root hair rhamnogalacturonan I contains more neutral oligosaccharide side chains than its root counterpart. At least 90% of the glucuronic acid is 4-O-methylated in root glucuronoxylan. Only 50% of this glycose is 4-O-methylated in the root hair counterpart. Mono O-acetylated fucose-containing subunits account for at least 60% of the neutral xyloglucan from root and root hair walls. By contrast, a galacturonic acid-containing xyloglucan was detected only in root hair cell walls. Soybean homologs of the Arabidopsis xyloglucan-specific galacturonosyltransferase are highly expressed only in root hairs. A mannose-rich polysaccharide was also detected only in root hair cell walls. Our data demonstrate that the walls of tip-growing root hairs cells have structural features that distinguish them from the walls of other roots cells. Springer-Verlag Berlin Heidelberg, 2015 Arabinogalactan protein (AGP) nationallicence Cell wall nationallicence Extensin nationallicence Hemicellulose nationallicence Pectin nationallicence Root nationallicence Root hair nationallicence AG : Arabinogalactan protein nationallicence CSLD : Cellulose synthase-like d nationallicence Dp : Degree of polymerization nationallicence MALDI-TOF MS : Matrix assisted laser desorption time of flight mass spectrometry nationallicence RG-I : Rhamnogalacturonan I nationallicence RG-II : Rhamnogalacturonan II nationallicence XEG : Xyloglucan-specific endoglucanase nationallicence XUT : Xyloglucan-specific galacturonosyltransferase nationallicence XXT : Xyloglucan-specific xylosyltransferase nationallicence XyGO : Xyloglucan oligosaccharide nationallicence Muszyński Artur Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut O'Neill Malcolm Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Ramasamy Easwaran Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Pattathil Sivakumar Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Avci Utku Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Peña Maria Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Libault Marc Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut Hossain Md Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut Brechenmacher Laurent Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut York William Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Barbosa Rommel Instituto de Informática, Universidade Federal de Goiás, 74001-970, Goiânia, Brazil aut Hahn Michael Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Stacey Gary Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut Carlson Russell Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1123-1138 0032-0935 242:5<1123 2015 242 425 https://doi.org/10.1007/s00425-015-2344-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2344-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muszyński Artur Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- O'Neill Malcolm Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Ramasamy Easwaran Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Pattathil Sivakumar Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Avci Utku Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Peña Maria Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Libault Marc Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Hossain Md Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Brechenmacher Laurent Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- York William Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Barbosa Rommel Instituto de Informática, Universidade Federal de Goiás, 74001-970, Goiânia, Brazil aut NATIONALLICENCE 700 1- Hahn Michael Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Stacey Gary Divisions of Plant Science and Biochemistry, National Center for Soybean Biotechnology, University of Missouri, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Carlson Russell Complex Carbohydrate Research Center, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1123-1138 0032-0935 242:5<1123 2015 242 425