caa a22 4500 606208380 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2393-2 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2393-2 Tracking transience: a method for dynamic monitoring of biological events in Arabidopsis thaliana biosensors [Elektronische Daten] [George Hines, Cyrus Modavi, Keni Jiang, Andrew Packard, Kameshwar Poolla, Lewis Feldman] Main conclusion : The activation and level of expression of an endogenous, stress-responsive biosensor (bioreporter) can be visualized in real-time and non-destructively using highly accessible equipment (fluorometer). Biosensor output can be linked to computer-controlled systems to enable feedback-based control of a greenhouse environment. Today's agriculture requires an ability to precisely and rapidly assess the physiological stress status of plants in order to optimize crop yield. Here we describe the implementation and utility of a detection system based on a simple fluorometer design for real-time, continuous, and non-destructive monitoring of a genetically engineered biosensor plant. We report the responses to heat stress of Arabidopsis thaliana plants expressing a Yellow Fluorescent Protein bioreporter under the control of the DREB2A temperature-sensing promoter. Use of this bioreporter provides the ability to identify transient and steady-state behavior of gene activation in response to stress, and serves as an interface for novel experimental protocols. Models identified through such experiments inform the development of computer-based feedback control systems for the greenhouse environment, based on in situ monitoring of mature plants. More broadly, the work here provides a basis for informing biologists and engineers about the kinetics of bioreporter constructs, and also about ways in which other fluorescent protein constructs could be integrated into automated control systems. Springer-Verlag Berlin Heidelberg, 2015 Bioreporter nationallicence Fluorescent proteins nationallicence Plant stress nationallicence DREB2A nationallicence Arabidopsis nationallicence Hines George Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut Modavi Cyrus Department of Bioengineering, University of California, 94720-3102, Berkeley, CA, USA aut Jiang Keni Department of Plant and Microbial Biology, University of California, 94720-3102, Berkeley, CA, USA aut Packard Andrew Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut Poolla Kameshwar Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut Feldman Lewis Department of Plant and Microbial Biology, University of California, 94720-3102, Berkeley, CA, USA aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1251-1261 0032-0935 242:5<1251 2015 242 425 https://doi.org/10.1007/s00425-015-2393-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2393-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hines George Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Modavi Cyrus Department of Bioengineering, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Jiang Keni Department of Plant and Microbial Biology, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Packard Andrew Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Poolla Kameshwar Department of Mechanical Engineering, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Feldman Lewis Department of Plant and Microbial Biology, University of California, 94720-3102, Berkeley, CA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1251-1261 0032-0935 242:5<1251 2015 242 425