caa a22 4500 606208402 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2354-9 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2354-9 Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense [Elektronische Daten] [Shigeto Morita, Yuki Yamashita, Masayoshi Fujiki, Rie Todaka, Yuri Nishikawa, Ayaka Hosoki, Chisato Yabe, Jun'ichi Nakamura, Kazuyoshi Kawamura, I. Suwastika, Masa Sato, Takehiro Masumura, Yasunari Ogihara, Kunisuke Tanaka, Shigeru Satoh] Main Conclusion : A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in developing and mature seeds. Seed tissues undergo drastic water loss at the late stage of seed development, and thus need to tolerate oxidative injuries associated with desiccation. We previously found a rice glutaredoxin isoform, OsGrxC2;2, as a gene expressed abundantly in developing seeds. Since glutaredoxin is involved in antioxidant defense, in the present study we investigated the subcellular localization and expression profile of OsGrxC2;2 and whether OsGrxC2;2 has a role in the defense against reactive oxygen species. Western blotting and immunohistochemistry revealed that the OsGrxC2;2 protein accumulated at a high level in the embryo and aleurone layers of developing and mature seeds. The OsGrxC2;2 in developing seeds was particularly localized to aleurone grains, which are storage organelles derived from vacuoles. Overexpression of OsGrxC2;2 resulted in an enhanced tolerance to menadione in yeast and methyl viologen in green leaves of transgenic rice plants. These results suggest that OsGrxC2;2 participates in the defense against oxidative stress in developing and mature seeds. Springer-Verlag Berlin Heidelberg, 2015 Aleurone layers nationallicence Antioxidative defense nationallicence Glutaredoxin nationallicence Oryza nationallicence Oxidative stress nationallicence AsA : Ascorbate nationallicence CaMV : Cauliflower mosaic virus nationallicence DAF : Days after flowering nationallicence DAI : Days after the onset of imbibition nationallicence DHAR : Dehydroascorbate reductase nationallicence ER : Endoplasmic reticulum nationallicence GFP : Green fluorescent protein nationallicence Grx : Glutaredoxin nationallicence GSH : Glutathione nationallicence Prx : Peroxiredoxin nationallicence PS : Photosystem nationallicence RFP : Red fluorescent protein nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence Trx : Thioredoxin nationallicence Morita Shigeto Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Yamashita Yuki Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Fujiki Masayoshi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Todaka Rie Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Nishikawa Yuri Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Hosoki Ayaka Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Yabe Chisato Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Nakamura Jun'ichi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Kawamura Kazuyoshi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Suwastika I. Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Sato Masa Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Masumura Takehiro Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Ogihara Yasunari Kihara Institute for Biological Research, Yokohama City University, Totsuka, 244-0813, Yokohama, Japan aut Tanaka Kunisuke Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Satoh Shigeru Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1195-1206 0032-0935 242:5<1195 2015 242 425 https://doi.org/10.1007/s00425-015-2354-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2354-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morita Shigeto Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamashita Yuki Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Fujiki Masayoshi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Todaka Rie Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Nishikawa Yuri Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Hosoki Ayaka Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Yabe Chisato Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Nakamura Jun'ichi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Kawamura Kazuyoshi Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Suwastika I. Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Sato Masa Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Masumura Takehiro Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Ogihara Yasunari Kihara Institute for Biological Research, Yokohama City University, Totsuka, 244-0813, Yokohama, Japan aut NATIONALLICENCE 700 1- Tanaka Kunisuke Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 700 1- Satoh Shigeru Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, 606-8522, Kyoto, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1195-1206 0032-0935 242:5<1195 2015 242 425