caa a22 4500 606208410 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00425-015-2351-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2351-z Durum wheat dehydrin (DHN-5) confers salinity tolerance to transgenic Arabidopsis plants through the regulation of proline metabolism and ROS scavenging system [Elektronische Daten] [Walid Saibi, Kaouthar Feki, Rihem Ben Mahmoud, Faiçal Brini] Main conclusion : The wheat dehydrin (DHN-5) gives birth to salinity tolerance to transgenic Arabidopsis plants by the regulation of proline metabolism and the ROS scavenging system. Dehydrins (DHNs) are involved in plant abiotic stress tolerance. In this study, we reported that salt tolerance of transgenic Arabidopsis plants overexpressing durum wheat dehydrin (DHN-5) was closely related to the activation of the proline metabolism enzyme (P5CS) and some antioxidant biocatalysts. Indeed, DHN-5 improved P5CS activity in the transgenic plants generating a significant proline accumulation. Moreover, salt tolerance of Arabidopsis transgenic plants was accompanied by an excellent activation of antioxidant enzymes like catalase (CAT), superoxide dismutase (SOD) and peroxide dismutase (POD) and generation of a lower level of hydrogen peroxide (H2O2) in leaves compared to the wild-type plants. The enzyme activities were enhanced in these transgenic plants in the presence of exogenous proline. Nevertheless, proline accumulation was slightly reduced in transgenic plants promoting chlorophyll levels. All these results suggest the crucial role of DHN-5 in response to salt stress through the activation of enzymes implicated in proline metabolism and in ROS scavenging enzymes. Springer-Verlag Berlin Heidelberg, 2015 Chlorophyll nationallicence Proline nationallicence P5CS activity nationallicence Salinity tolerance nationallicence Transgenic Arabidopsis plants nationallicence Wheat dehydrin (DHN-5), antioxidant enzymes nationallicence Saibi Walid Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut Feki Kaouthar Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut Ben Mahmoud Rihem Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut Brini Faiçal Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1187-1194 0032-0935 242:5<1187 2015 242 425 https://doi.org/10.1007/s00425-015-2351-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2351-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saibi Walid Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut NATIONALLICENCE 700 1- Feki Kaouthar Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut NATIONALLICENCE 700 1- Ben Mahmoud Rihem Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut NATIONALLICENCE 700 1- Brini Faiçal Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax-University of Sfax, B.P 1177, 3018, Sfax, Tunisia aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/5(2015-11-01), 1187-1194 0032-0935 242:5<1187 2015 242 425