caa a22 4500 606208453 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00425-015-2312-6 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2312-6 Walters Christina USDA-ARS, National Center for Genetic Resources Preservation, 1111 South Mason Street, 80521, Fort Collins, CO, USA aut Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss [Elektronische Daten] [Christina Walters] Main conclusion : Discrete categories of seed physiology can be explained through a unified concept of the structural and molecular mobility responses within cells to drying. Tolerance of desiccation is typically described by a threshold or low water content limit to survival. This convention provides fairly good distinction between orthodox and recalcitrant seeds, which show thresholds of less than about 0.07 and greater than about 0.2gH2OgDW−1, respectively. Threshold water contents, however, are not direct measures of the intensity of water stress tolerated by seeds, nor are they measures of cell response to water stress. More direct criteria, that accommodate both spatial and temporal effects of water loss, are required to explain variation of desiccation tolerance and longevity in seeds from diverse genetic backgrounds and growth conditions. This essay presents the argument that changes in cellular volume directly quantify primary responses to desiccating stress in a context that also links damage, as cellular constituents compress, and protection, as compressed molecules form stabilizing structure. During desiccation, fluid cytoplasm solidifies, and the newly formed spatial relationships among molecules determine whether and how long viability is maintained. The diversity of seed behaviors suggests complexity and opportunity to discover molecules and mechanisms that regulate survival and perception of time in cells that lack metabolic function. Springer-Verlag Berlin Heidelberg (outside the USA), 2015 Cell shrinkage nationallicence Configurational entropy nationallicence Desiccation nationallicence Desiccation tolerance nationallicence Glass formation nationallicence Plasmolysis nationallicence Temperature nationallicence Threshold nationallicence DW : Dry weight nationallicence LN : Liquid nitrogen nationallicence Planta Springer Berlin Heidelberg 242/2(2015-08-01), 397-406 0032-0935 242:2<397 2015 242 425 https://doi.org/10.1007/s00425-015-2312-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2312-6 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Walters Christina USDA-ARS, National Center for Genetic Resources Preservation, 1111 South Mason Street, 80521, Fort Collins, CO, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/2(2015-08-01), 397-406 0032-0935 242:2<397 2015 242 425