caa a22 4500 606208461 CHVBK 20210128101022.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00425-015-2281-9 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2281-9 Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana [Elektronische Daten] [Steven Hand, Michael Menze] Main conclusion : We have evaluated the endogenous expression and molecular properties of selected Group 3 LEA proteins from Artemia franciscana , and the capacity of selected Groups 1 and 3 proteins transfected into various desiccation-sensitive cell lines to improve tolerance to drying. Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons—exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages of the life cycle. Such is the case for gastrula-stage embryos of the brine shrimp, Artemia franciscana. Physiological and biochemical responses to desiccation are central for survival and are multifaceted. This review will evaluate the impact of multiple late embryogenesis abundant proteins originating from A. franciscana, together with the non-reducing sugar trehalose, on prevention of desiccation damage at multiple levels of biological organization. Survivorship of desiccation-sensitive cells during water stress can be improved by use of the above protective agents, coupled to metabolic preconditioning and rapid cell drying. However, obtaining long-term stability of cells in the dried state at room temperature has not been accomplished and will require continued efforts on both the physicochemical and biological fronts. Springer-Verlag Berlin Heidelberg, 2015 Anhydrobiosis nationallicence Diapause nationallicence Intrinsically disordered proteins nationallicence Late embryogenesis abundant proteins nationallicence Trehalose nationallicence LEA : Late embryogenesis abundant nationallicence ROS : Reactive oxygen species nationallicence T g : Glass transition temperature nationallicence GFP : Green fluorescent protein nationallicence TFE : Trifluoroethanol nationallicence PFK : Phosphofructokinase nationallicence CS : Citrate synthase nationallicence LDH : Lactate dehydrogenase nationallicence AMPK : AMP activated protein kinase nationallicence AICAR : 5-Amino-4-imidazole carboxamide ribonucleoside nationallicence ZMP : 5-Amino-4-imidazole carboxamide ribonucleoside monophosphate nationallicence Hand Steven Division of Cellular, Developmental and Integrative Biology, Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Menze Michael Department of Biological Science, Eastern Illinois University, 61920, Charleston, IL, USA aut Planta Springer Berlin Heidelberg 242/2(2015-08-01), 379-388 0032-0935 242:2<379 2015 242 425 https://doi.org/10.1007/s00425-015-2281-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2281-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hand Steven Division of Cellular, Developmental and Integrative Biology, Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Menze Michael Department of Biological Science, Eastern Illinois University, 61920, Charleston, IL, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/2(2015-08-01), 379-388 0032-0935 242:2<379 2015 242 425