caa a22 4500 606208496 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00425-015-2300-x doi (NATIONALLICENCE)springer-10.1007/s00425-015-2300-x The C. elegans dauer larva as a paradigm to study metabolic suppression and desiccation tolerance [Elektronische Daten] [Cihan Erkut, Teymuras Kurzchalia] Main conclusion : The hypometabolic, stress-resistant dauer larva of Caenorhabditis elegans serves as an excellent model to study the molecular mechanisms of desiccation tolerance, such as maintenance of membrane organization, protein folding, xenobiotic and ROS detoxification in the dry state. Many organisms from diverse taxa of life have the remarkable ability to survive extreme desiccation in the nature by entering an ametabolic state known as anhydrobiosis (life without water). The hallmark of the anhydrobiotic state is the achievement and maintenance of an exceedingly low metabolic rate, as well as preservation of the structural integrity of the cell. Although described more than three centuries ago, the biochemical and biophysical mechanisms underlying this phenomenon are still not fully comprehended. This is mainly due to the fact that anhydrobiosis in animals was studied using non-model organisms, which are very difficult, if not impossible, to manipulate at the molecular level. Recently, we introduced the roundworm (nematode) Caenorhabditis elegans as a model for anhydrobiosis. Taking advantage of powerful genetic, biochemical and biophysical tools, we investigated several aspects of anhydrobiosis in a particular developmental stage (the dauer larva) of this organism. First, our studies allowed confirming the previously suggested role of the disaccharide trehalose in the preservation of lipid membranes. Moreover, in addition to known pathways such as reactive oxygen species defense, heat-shock and intrinsically disordered protein expression, evidence for some novel strategies of anhydrobiosis has been obtained. These are increased glyoxalase activity, polyamine and polyunsaturated fatty acid biosynthesis. All these pathways may constitute a generic toolbox of anhydrobiosis, which is possibly conserved between animals and plants. Springer-Verlag Berlin Heidelberg, 2015 Ametabolism nationallicence Anhydrobiosis nationallicence Drought tolerance nationallicence Hypometabolism nationallicence Nematode nationallicence Trehalose nationallicence Cas : CRISPR-associated nationallicence CRISPR : Clustered, regularly interspaced, short palindromic repeats nationallicence dsRNA : Double-stranded RNA nationallicence GFP : Green fluorescent protein nationallicence HSP : Heat-shock protein nationallicence IDP : Intrinsically disordered protein nationallicence LEA : Late embryogenesis abundant nationallicence PC : Phosphatidylcholine nationallicence PE : Phosphatidylethanolamine nationallicence PUFA : Polyunsaturated fatty acid nationallicence RH : Relative humidity nationallicence RNAi : RNA interference nationallicence ROS : Reactive oxygen species nationallicence TGF-β : Transforming growth factor beta nationallicence TPS : Trehalose 6-phosphate synthase nationallicence TRPV : Transient receptor potential, vanilloid type nationallicence Erkut Cihan Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany aut Kurzchalia Teymuras Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany aut Planta Springer Berlin Heidelberg 242/2(2015-08-01), 389-396 0032-0935 242:2<389 2015 242 425 https://doi.org/10.1007/s00425-015-2300-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2300-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erkut Cihan Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Kurzchalia Teymuras Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/2(2015-08-01), 389-396 0032-0935 242:2<389 2015 242 425