caa a22 4500 606208550 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00425-015-2284-6 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2284-6 Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki [Elektronische Daten] [Rie Hatanaka, Oleg Gusev, Richard Cornette, Sachiko Shimura, Shingo Kikuta, Jun Okada, Takashi Okuda, Takahiro Kikawada] Main conclusion : In the anhydrobiotic midge Polypedilum vanderplanki , LEA family proteins are likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration. The larvae of the anhydrobiotic midge, P. vanderplanki, which can tolerate almost complete desiccation, accumulate late embryogenesis abundant (LEA) proteins in response to drying. Using complete genome data of the midge, we have identified 27 PvLea1-like genes based on the similarity to previously characterized PvLea1 gene belonging to group 3 LEA proteins. Generally, group 3 LEA proteins are characterized by several repetitions of an 11-mer motif. However, some PvLea genes lack the canonical motif in their sequences. We performed the detailed characterization of all 27 PvLea genes in terms of biochemical and biophysical properties and conserved motifs. The motif analysis among their amino acid sequences revealed that all 27 PvLEA proteins have at least one of two types of motifs (motif 1: G AKDTTKEKLGE AKDATAEKLG or motif 2: KD ILExAKDKLxD AKDAVKEKL), indicating the presence of at least two repeated 11-mer LEA motifs. Most of PvLEA proteins were localized to the cytosol. We also performed quantitative real-time PCR of all 27 PvLea genes in detail during the process of desiccation and rehydration. The expression of these genes was upregulated at the beginning of dehydration, the latter phase of the desiccation process and on rehydration process. These data suggested that each LEA protein is likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration. Springer-Verlag Berlin Heidelberg, 2015 Anhydrobiosis nationallicence Desiccation tolerance nationallicence Gene expression nationallicence Late embryogenesis abundant (LEA) protein nationallicence Polypedilum vanderplanki nationallicence Subcellular localization nationallicence CHO : Chinese hamster ovary nationallicence ER : Endoplasmic reticulum nationallicence LEA : Late embryogenesis abundant nationallicence GFP : Green fluorescent protein nationallicence GRAVY : Grand average of hydropathy nationallicence qRT-PCR : Quantitative real-time PCR nationallicence pI : Isoelectric point nationallicence Hatanaka Rie National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Gusev Oleg National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Cornette Richard National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Shimura Sachiko National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Kikuta Shingo National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Okada Jun National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Okuda Takashi National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Kikawada Takahiro National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut Planta Springer Berlin Heidelberg 242/2(2015-08-01), 451-459 0032-0935 242:2<451 2015 242 425 https://doi.org/10.1007/s00425-015-2284-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2284-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatanaka Rie National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Gusev Oleg National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Cornette Richard National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Shimura Sachiko National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Kikuta Shingo National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Okada Jun National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Okuda Takashi National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 700 1- Kikawada Takahiro National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/2(2015-08-01), 451-459 0032-0935 242:2<451 2015 242 425