caa a22 4500 606208569 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2157-4 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2157-4 A putative role for γ-aminobutyric acid (GABA) in vascular development in pine seedlings [Elektronische Daten] [Juan Molina-Rueda, María Pascual, José Pissarra, Fernando Gallardo] Main conclusion : A model for GABA synthesis in stems of pine seedlings is proposed. The localization of GABA in differentiating tracheids suggests a link between GABA production and vascular development. Abstract: γ-aminobutyric acid (GABA) is a non-proteinogenic amino acid present in both prokaryotic and eukaryotic organisms. GABA plays a fundamental role as a signal molecule in the central nervous system in animals. In plants, GABA has been correlated with cellular elongation, plant development, gene expression regulation, synthesis of ethylene and other hormones, and signaling. Considering the physiological importance of GABA in plants, the lack of works about GABA localization in this kingdom seems surprising. In this work, the immunolocalization of GABA in root and hypocotyl during seedling development and in bent stem showing compression xylem has been studied. In the seedling root, the GABA signal was very high and restricted to the stele supporting previous evidences indicating a potential role for this amino acid in root growth and nutrient transport. In hypocotyl, GABA was localized in vascular tissues, including differentiating xylem, ray parenchyma and epithelial resin duct cells, drawing also a role for GABA in vascular development, communication and defense. During the production of compression wood, a special lignified wood produced when the stem loss its vertical position, a clear GABA signal was found in the new differentiating xylem cells showing a gradient-like pattern with higher signal in less differentiated elements. The results are in accordance with a previous work indicating that glutamate decarboxylase and GABA production are associated to vascular differentiation in pine Molina-Rueda et al. (Planta 232: 1471-1483, 2010). A model for GABA synthesis in vascular differentiation, communication, and defense is proposed in the stem of pine seedlings. Springer-Verlag Berlin Heidelberg, 2014 γ-aminobutyric acid nationallicence Differentiating tracheids nationallicence Pine seedling nationallicence Radial parenchyma nationallicence Reaction wood nationallicence Resin duct cells nationallicence Root nationallicence Stele nationallicence Stem nationallicence Vascular development nationallicence GABA : γ-aminobutyric acid nationallicence GAD : Glutamate decarboxylase nationallicence Molina-Rueda Juan Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut Pascual María Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut Pissarra José Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal aut Gallardo Fernando Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 257-267 0032-0935 241:1<257 2015 241 425 https://doi.org/10.1007/s00425-014-2157-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2157-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Molina-Rueda Juan Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut NATIONALLICENCE 700 1- Pascual María Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut NATIONALLICENCE 700 1- Pissarra José Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal aut NATIONALLICENCE 700 1- Gallardo Fernando Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 257-267 0032-0935 241:1<257 2015 241 425